Questiomycin A (Synonyms: Phx-3)

Name: Questiomycin A
Brand: MedChemExpress
SKU: HY-N8439
Rating: 4.5 (1 reviews)

Cat. No.: HY-N8439 Purity: 98.20%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Questiomycin A (Phx-3) is a GRP78 (cytoprotective endoplasmic reticulum chaperone) degrader and enhances the anticancer activity of Sorafenib. Questiomycin A is also an antimicrobial/antibiotic that can be obtained from the metabolite of Pseudomonas chlororaphis HT66. Questiomycin A can be used in research on biological control of cancer and plant diseases.

For research use only. We do not sell to patients.

Questiomycin A Chemical Structure

CAS No. : 1916-59-2

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

GRP78, bacterial^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
CCRF-CEM	IC₅₀	2 μM Compound: 1	Compound concentration required to reduce the cell proliferation of human acute T-lymphoblastic leukemia CCRF-CEM cells by 50%, using MTT method Compound concentration required to reduce the cell proliferation of human acute T-lymphoblastic leukemia CCRF-CEM cells by 50%, using MTT method	[PMID: 12431048]
CCRF-SB	IC₅₀	1 μM Compound: 1	Compound concentration required to reduce the cell proliferation of human acute B-lymphoblastic leukemia CCRF-SB cells by 50%, using MTT method Compound concentration required to reduce the cell proliferation of human acute B-lymphoblastic leukemia CCRF-SB cells by 50%, using MTT method	[PMID: 12431048]
HeLa	IC₅₀	2.7 μM Compound: 1	Compound concentration required to reduce the cell proliferation of cervix carcinoma Hela cells by 50%, using MTT method Compound concentration required to reduce the cell proliferation of cervix carcinoma Hela cells by 50%, using MTT method	[PMID: 12431048]
HEp-2	IC₅₀	1.8 μM Compound: 1	Compound concentration required to reduce the cell proliferation of larynx carcinoma cells by 50%, using MTT method Compound concentration required to reduce the cell proliferation of larynx carcinoma cells by 50%, using MTT method	[PMID: 12431048]

In Vitro

Questiomycin A (3 µM; 24 h) induces cell death and downregulates GRP78 protein expression in HepG2 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HepG2 cells
Concentration:	3 µM
Incubation Time:	24 h
Result:	Suppressed more than 50% of the GRP78 expression and induced cell death.

In Vivo

Questiomycin A (10 mg/kg; i.p.; once a day for 8 days) enhances the antitumor effect of Sorafenib by stimulating the downregulation of GRP78 in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nude mice (HepG2 cells xenograft model)^[1].
Dosage:	10 mg/kg
Administration:	Intraperitoneal injection; once a day; 8 days.
Result:	Stimulated Sorafenib-dependent antitumor activity.

Molecular Weight

212.20

Formula

C₁₂H₈N₂O₂

CAS No.

1916-59-2

Appearance

Solid

Color

Brown to black

SMILES

O=C1C(N)=CC2=NC3=C(C=CC=C3)OC2=C1

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (58.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7125 mL	23.5627 mL	47.1254 mL
5 mM	0.9425 mL	4.7125 mL	9.4251 mL
10 mM	0.4713 mL	2.3563 mL	4.7125 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.20%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) HNMR (253 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Machihara K, et al. Questiomycin A stimulates sorafenib-induced cell death via suppression of glucose-regulated protein 78. Biochem Biophys Res Commun. 2017 Oct 7;492(1):33-40. [Content Brief]

[2]. Guo S, et al. Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66. J Agric Food Chem. 2022 Jun 29;70(25):7742-7750. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.7125 mL	23.5627 mL	47.1254 mL	117.8134 mL
	5 mM	0.9425 mL	4.7125 mL	9.4251 mL	23.5627 mL
	10 mM	0.4713 mL	2.3563 mL	4.7125 mL	11.7813 mL
	15 mM	0.3142 mL	1.5708 mL	3.1417 mL	7.8542 mL
	20 mM	0.2356 mL	1.1781 mL	2.3563 mL	5.8907 mL
	25 mM	0.1885 mL	0.9425 mL	1.8850 mL	4.7125 mL
	30 mM	0.1571 mL	0.7854 mL	1.5708 mL	3.9271 mL
	40 mM	0.1178 mL	0.5891 mL	1.1781 mL	2.9453 mL
	50 mM	0.0943 mL	0.4713 mL	0.9425 mL	2.3563 mL

Questiomycin A Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Questiomycin A (Synonyms: Phx-3)

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Questiomycin A Related Antibodies

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Molarity Calculator

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Complete Stock Solution Preparation Table

Questiomycin A Related Classifications

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