(R)-Sulforaphane (Synonyms: L-Sulforaphane)

Name: (R)-Sulforaphane
Brand: MedChemExpress
SKU: HY-13755A
Rating: 4.5 (1 reviews)

Cat. No.: HY-13755A Purity: 99.48%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

(R)-Sulforaphane (L-Sulforaphane) is a orally active, potent inducer of the Keap1/Nrf2/ARE pathway, exhibiting antioxidant and anticancer activities. (R)-Sulforaphane primarily functions by upregulating phase II detoxifying enzymes in cells, aiding in the removal of carcinogens and combating oxidative stress. (R)-Sulforaphane is capable of modulating gene expression, influencing various signaling pathways, including Nrf2, NF-κB, and AP-1. (R)-Sulforaphane can be used in studies of tumor biology, antioxidant defense mechanisms, as well as inflammation and immune responses.

For research use only. We do not sell to patients.

(R)-Sulforaphane Chemical Structure

CAS No. : 142825-10-3

Size	Price	Stock	Quantity
5 mg	USD 168	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 286	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of (R)-Sulforaphane:

Sulforaphane In-stock

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Bax

Procaspase-3

NF-κB

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	19.6 μM Compound: 2-RS	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25299679]
MRC5	IC₅₀	46.58 μM Compound: 2-RS	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25299679]

In Vitro

(R)-Sulforaphane (10 μM, 24 hours) pre-treatment of H9c2 cells reduces the number of apoptotic cells, decreases the expression of pro-apoptotic proteins (Bax, caspase-3, cytochrome c), and counteracts the increase in mitochondrial membrane potential induced by Doxorubicin (HY-15142A) (1 μM, 2 hours)^[4].
(R)-Sulforaphane (10 μM, 2 or 24 hours) pre-treatment enhances the expression of HO-1 in H9c2 cells and reduces the levels of ROS within the mitochondria (measured using MitoSOX Red reagent) induced by Doxorubicin (1 μM, 2 or 24 hours)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[5]

Cell Line:	MCF7 cells
Concentration:	20 μM
Incubation Time:	72h
Result:	Increased the most the NQO1 transcript and protein levels in MCF7 cells. Increased of Nrf2 transcript was observed in MCF7 cells. Reduced transcription and protein of AhR gene in MCF7 cells.

Apoptosis Analysis^[4]

Cell Line:	H9c2 cells
Concentration:	L-sulforaphane or D,L-sulforaphane: 10 μM; Doxorubicin: 1 μM
Incubation Time:	L-sulforaphane or D,L-sulforaphane: 2h; Doxorubicin: 2, 24h
Result:	Protected the H9c2 cells against doxorubicin-induced cell death. Increased cell viability in a dose-dependent manner. Significantly reduced the number of apoptotic cells treated with Doxorubicin.

RT-PCR^[4]

Cell Line:	H9c2 cells
Concentration:	L-sulforaphane or D,L-sulforaphane: 10 μM; Doxorubicin: 1 μM
Incubation Time:	L-sulforaphane or D,L-sulforaphane: 2h, 24h; Doxorubicin: 2h, 24h
Result:	Induced heme oxygenase-1 (HO-1) mRNA expression in a dose-dependent manner.

Western Blot Analysis^[4]

Cell Line:	H9c2 cells
Concentration:	L-sulforaphane or D,L-sulforaphane: 10 μM; Doxorubicin: 1 μM
Incubation Time:	L-sulforaphane or D,L-sulforaphane: 2h; Doxorubicin: 2, 24h
Result:	Prevented the release of cytochrome c into the cytosol. Prevented the translocation of Bax into the cytosol. Attenuated the doxorubicin-induced increase in the levels of cleaved caspase-3. Induced a significant increase in HO-1 protein expression. Induced a significantly higher level of Nrf2 expression in the nucleus compared to the cytoplasm.

Molecular Weight

177.29

Formula

C₆H₁₁NOS₂

CAS No.

142825-10-3

Appearance

Liquid (Density: 1.17±0.1 g/cm³)

Color

Colorless to light yellow

SMILES

O=[S@@](CCCCN=C=S)C

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (564.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6405 mL	28.2024 mL	56.4048 mL
5 mM	1.1281 mL	5.6405 mL	11.2810 mL
10 mM	0.5640 mL	2.8202 mL	5.6405 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.48%

Select Batch:

Data Sheet (276 KB) SDS (644 KB)

COA (243 KB) HNMR (132 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. De Nicola GR, et al. Novel gram-scale production of enantiopure R-sulforaphane from Tuscan black kale seeds. Molecules. 2014 May 27;19(6):6975-86. [Content Brief]

[2]. Abdull Razis AF, et al. The natural chemopreventive phytochemical R-sulforaphane is a far more potent inducer of the carcinogen-detoxifying enzyme systems in rat liver and lung than the S-isomer. Int J Cancer. 2011 Jun 15;128(12):2775-82. [Content Brief]

[3]. Gamet-Payrastre L, et al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res. 2000;60(5):1426-1433. [Content Brief]

[4]. Li B, et al. Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells. Int J Mol Med. 2015 Jul;36(1):53-64. doi: 10.3892/ijmm.2015.2199. Epub 2015 Apr 28. [Content Brief]

[5]. Licznerska B, et al. R-sulforaphane modulates the expression profile of AhR, ERα, Nrf2, NQO1, and GSTP in human breast cell lines. Mol Cell Biochem. 2021;476(2):525-533. [Content Brief]

[6]. Zhang Y, et al. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornylisothiocyanates. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3147-50. [Content Brief]

[7]. Chen X, et al. Activation of Nrf2 by Sulforaphane Inhibits High Glucose-Induced Progression of PancreaticCancer via AMPK Dependent Signaling. ell Physiol Biochem. 2018;50(3):1201-1215. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6405 mL	28.2024 mL	56.4048 mL	141.0119 mL
	5 mM	1.1281 mL	5.6405 mL	11.2810 mL	28.2024 mL
	10 mM	0.5640 mL	2.8202 mL	5.6405 mL	14.1012 mL
	15 mM	0.3760 mL	1.8802 mL	3.7603 mL	9.4008 mL
	20 mM	0.2820 mL	1.4101 mL	2.8202 mL	7.0506 mL
	25 mM	0.2256 mL	1.1281 mL	2.2562 mL	5.6405 mL
	30 mM	0.1880 mL	0.9401 mL	1.8802 mL	4.7004 mL
	40 mM	0.1410 mL	0.7051 mL	1.4101 mL	3.5253 mL
	50 mM	0.1128 mL	0.5640 mL	1.1281 mL	2.8202 mL
	60 mM	0.0940 mL	0.4700 mL	0.9401 mL	2.3502 mL
	80 mM	0.0705 mL	0.3525 mL	0.7051 mL	1.7626 mL
	100 mM	0.0564 mL	0.2820 mL	0.5640 mL	1.4101 mL

(R)-Sulforaphane Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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(R)-Sulforaphane (Synonyms: L-Sulforaphane)

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(R)-Sulforaphane Related Antibodies

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Complete Stock Solution Preparation Table

(R)-Sulforaphane Related Classifications

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