(±)-H3RESCA-TFP (Synonyms: (±)-H3L28)

Name: (±)-H3RESCA-TFP
Brand: MedChemExpress
SKU: HY-133513
Rating: 4.5 (1 reviews)

Cat. No.: HY-133513 Purity: 98.15%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

(±)-H3RESCA-TFP ((±)-H3L28) is a tetrafluorophenyl ester derivative of restrained complexing agent (RESCA). (±)-H3RESCA-TFP can be used to conjugate the chelator with a biomolecule via amine coupling (e.g., N terminus and/or the ε-amino groups of lysine).

For research use only. We do not sell to patients.

(±)-H3RESCA-TFP Chemical Structure

CAS No. : 1919794-40-3

Size	Price	Stock	Quantity
5 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

In Vitro

The aluminum fluoride restrained complexing agent (Al¹⁸F-RESCA) method combines the chemical advantages of a chelator-based radiolabeling method with the unique physical properties of the radionuclide of choice, fluorine-18. Proteins of interest can be conjugated to RESCA via amine coupling using (±)-H3RESCA-TFP. Al¹⁸F-labeled proteins can be obtained with moderate (12-17 GBq/µmol) to good (80-85 GBq/µmol) apparent molar activity, depending on the starting activity of ¹⁸F^-^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

584.51

Formula

C₂₇H₂₈F₄N₂O₈

CAS No.

1919794-40-3

Appearance

Solid

Color

White to off-white

SMILES

O=C(CC1=CC=C(CN(CC(O)=O)[C@@H]2CCCC[C@H]2N(CC(O)=O)CC(O)=O)C=C1)OC3=C(F)C(F)=CC(F)=C3F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (171.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7108 mL	8.5542 mL	17.1083 mL
5 mM	0.3422 mL	1.7108 mL	3.4217 mL
10 mM	0.1711 mL	0.8554 mL	1.7108 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.15%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (254 KB) HNMR (275 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Cleeren F, et al. Direct fluorine-18 labeling of heat-sensitive biomolecules for positron emission tomography imaging using the Al18F-RESCA method. Nat Protoc. 2018 Oct;13(10):2330-2347. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7108 mL	8.5542 mL	17.1083 mL	42.7709 mL
	5 mM	0.3422 mL	1.7108 mL	3.4217 mL	8.5542 mL
	10 mM	0.1711 mL	0.8554 mL	1.7108 mL	4.2771 mL
	15 mM	0.1141 mL	0.5703 mL	1.1406 mL	2.8514 mL
	20 mM	0.0855 mL	0.4277 mL	0.8554 mL	2.1385 mL
	25 mM	0.0684 mL	0.3422 mL	0.6843 mL	1.7108 mL
	30 mM	0.0570 mL	0.2851 mL	0.5703 mL	1.4257 mL
	40 mM	0.0428 mL	0.2139 mL	0.4277 mL	1.0693 mL
	50 mM	0.0342 mL	0.1711 mL	0.3422 mL	0.8554 mL
	60 mM	0.0285 mL	0.1426 mL	0.2851 mL	0.7128 mL
	80 mM	0.0214 mL	0.1069 mL	0.2139 mL	0.5346 mL
	100 mM	0.0171 mL	0.0855 mL	0.1711 mL	0.4277 mL

(±)-H3RESCA-TFP Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(±)-H3RESCA-TFP1919794-40-3(±)-H3L28OthersRESCAtetrafluorophenylε-aminolysinchelatorbiomoleculeamineInhibitorinhibitorinhibit

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(±)-H3RESCA-TFP (Synonyms: (±)-H3L28)

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(±)-H3RESCA-TFP Related Antibodies

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Complete Stock Solution Preparation Table

(±)-H3RESCA-TFP Related Classifications

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