Radioprotectin-1

Name: Radioprotectin-1
Brand: MedChemExpress
SKU: HY-114380
Rating: 4.5 (1 reviews)

Cat. No.: HY-114380 Purity: 99.30%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Radioprotectin-1 is a potent and specific nonlipid agonist of lysophosphatidic acid receptor 2 (LPA₂), with an EC₅₀ value of 25 nM for murine LPA₂ subtype.

For research use only. We do not sell to patients.

Radioprotectin-1 Chemical Structure

CAS No. : 1622006-09-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 396	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 396	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 370	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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LPAR1 LPAR2 LPAR3 LPAR5 S1PR1 S1PR2 S1PR3 S1PR4 S1PR5

Biological Activity
Purity & Documentation
References
Customer Review

Description

Radioprotectin-1 is a potent and specific nonlipid agonist of lysophosphatidic acid receptor 2 (LPA₂), with an EC₅₀ value of 25 nM for murine LPA₂ subtype^[1].

IC₅₀ & Target

EC50: 25 nM (murine LPA₂ subtype)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
MEF	EC₅₀	5.06 x 10^-6 μM Compound: 11d	Agonist activity at human LPA2 expressed in LPA1xLPA2 double knockout mouse MEF cells by Fura-2AM dye based Ca2+ mobilization assay Agonist activity at human LPA2 expressed in LPA1xLPA2 double knockout mouse MEF cells by Fura-2AM dye based Ca2+ mobilization assay	[PMID: 25100502]

In Vitro

Radioprotectin-1 is a potent agonist of LPA₂ with an EC₅₀ of 5 pM and functions as a full agonist at the human ortholog of LPA₂^[1].
Radioprotectin-1 (0-3 μM; 15 minutes) effectively reduces apoptosis induced by γ-irradiation and the radiomimetic drug Adriamycin in cells that expressed LPA₂ either endogenously or after transfection^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MEF cells , IEC-6 cells
Concentration:	0 μM, 0.1 μM, 0.3 μM, 1.0 μM,3 μM
Incubation Time:	15 minutes
Result:	Protected cells from apoptosis by γ-irradiation- and the radiomimetic chemotherapeutic Adiamycin.

In Vivo

Radioprotectin-1 is a high-potency specific agonist of the murine LPA₂ GPCR ^[1].
Radioprotectin-1 (0.1 mg/kg, 0.3 mg/kg; s.c.; every 12 hours; for 3 days) decreases the mortality of C57BL/6 mice in models of the hematopoietic acute radiation syndromes (HE-ARS) and gastrointestinal acute radiation syndromes (GI-ARS) ^[1].
Radioprotectin-1 exerts its radioprotective and radiomitigative action through specific activation of the upregulated LPA₂ GPCR in Lgr5⁺stem cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-10 weeks old C57BL/6 female mice bearing total body irradiation (TBI) ^[1]
Dosage:	0.1 mg/kg, 0.3 mg/kg
Administration:	Subcutaneous injection; every 12 hours; for 3 days
Result:	Decreased mortality of C57BL/6 mice in models of the HE-ARS and the GI-ARS.

Molecular Weight

486.92

Formula

C₂₃H₁₉ClN₂O₆S

CAS No.

1622006-09-0

Appearance

Solid

Color

White to light yellow

SMILES

O=C(O)C1=CC(Cl)=CC=C1S(=O)(NCCCCN(C(C2=CC=CC3=CC=CC4=C23)=O)C4=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (256.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0537 mL	10.2686 mL	20.5373 mL
5 mM	0.4107 mL	2.0537 mL	4.1075 mL
10 mM	0.2054 mL	1.0269 mL	2.0537 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.30%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (252 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Kuo B, et al. The LPA2 receptor agonist Radioprotectin-1 spares Lgr5-positive intestinal stem cells from radiation injury in murine enteroids. Cell Signal. 2018 Nov;51:23-33. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0537 mL	10.2686 mL	20.5373 mL	51.3431 mL
	5 mM	0.4107 mL	2.0537 mL	4.1075 mL	10.2686 mL
	10 mM	0.2054 mL	1.0269 mL	2.0537 mL	5.1343 mL
	15 mM	0.1369 mL	0.6846 mL	1.3692 mL	3.4229 mL
	20 mM	0.1027 mL	0.5134 mL	1.0269 mL	2.5672 mL
	25 mM	0.0821 mL	0.4107 mL	0.8215 mL	2.0537 mL
	30 mM	0.0685 mL	0.3423 mL	0.6846 mL	1.7114 mL
	40 mM	0.0513 mL	0.2567 mL	0.5134 mL	1.2836 mL
	50 mM	0.0411 mL	0.2054 mL	0.4107 mL	1.0269 mL
	60 mM	0.0342 mL	0.1711 mL	0.3423 mL	0.8557 mL
	80 mM	0.0257 mL	0.1284 mL	0.2567 mL	0.6418 mL
	100 mM	0.0205 mL	0.1027 mL	0.2054 mL	0.5134 mL