Animal-Free IL-30/IL-27A Protein, Human (His)

IL-30/IL-27A proteins cooperate with EBI3 to form IL-27, a key cytokine in innate immunity with pro- and anti-inflammatory properties. IL-27 regulates T helper cell development, inhibits T cell proliferation and affects various immune cells, affects CD4 T helper cell differentiation and promotes rapid clonal expansion. Animal-Free IL-30/IL-27A Protein, Human (His) is the recombinant human-derived animal-FreeIL-30/IL-27A protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-30/IL-27A Protein, Human (His) is 215 a.a., with molecular weight of ~25.47 kDa.

The IL-30/IL-27A Protein collaborates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine playing a pivotal role in innate immunity. Demonstrating both pro- and anti-inflammatory properties, IL-27 exerts multifaceted effects on immune function. It regulates T-helper cell development, inhibits T-cell proliferation, enhances cytotoxic T-cell activity, induces isotype switching in B-cells, and exerts diverse effects on innate immune cells. Among its target cells, CD4 T-helper cells differentiate into TH1, TH2, and TH17 cells under its influence. IL-27 prompts rapid clonal expansion of naive CD4 T-cells, synergizes with IL-12 for interferon-gamma production, and binds to the cytokine receptor WSX-1/TCCR for signal transduction. Potentiating the early phase of TH1 response, IL-27 suppresses TH2 and TH17 differentiation, impacting GATA3 expression and promoting TBX21/T-Bet activation via STAT1. In CD8 T-cells, it activates STATs and GZMB. Notably, IL-27 inhibits TH17 cell development and IL-17 production, suppressing pro-inflammatory cytokines, activating suppressors of cytokine signaling, and antagonizing the effects of cytokines like IL6. Beyond its immunomodulatory functions, IL-27 exhibits antitumor and antiangiogenic activities, inducing the production of antiangiogenic chemokines in endothelial cells and displaying antiviral properties by inhibiting HIV-1 replication. The heterodimeric structure of IL-27, formed with EBI3, is crucial for its functions and is known as interleukin IL-27.

Human

E. coli

C-His

Q8NEV9 (F29-P243)

246778  [NCBI]

MFPRPPGRPQLSLQELRREFTVSLHLARKLLSEVRGQAHRFAESHLPGVNLYLLPLGEQLPDVSLTFQAWRRLSDPERLCFISTTLQPFHALLGGLGTQGRWTNMERMQLWAMRLDLRDLQRHLRFQVLAAGFNLPEEEEEEEEEEEEERKGLLPGALGSALQGPAQVSWPQLLSTYRLLHSLELVLSRAVRELLLLSKAGHSVWPLGFPTLSPQP

Approximately 25.47 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.