Animal-Free IL-5 Protein, Human (His)

The IL-5 Protein, predominantly expressed by T-lymphocytes and NK cells, is crucial for eosinophil survival, differentiation, and chemotaxis. It also affects B-cells, inducing immunoglobulin production, growth, and differentiation. IL-5 Protein activates kinases like LYN, SYK, and JAK2, which signal through RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer and interacts with IL5RA and CSF2RB subunits. Animal-Free IL-5 Protein, Human (His) is the recombinant human-derived animal-FreeIL-5 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-5 Protein, Human (His) is 115 a.a., with molecular weight of ~14.09 kDa.

IL-5 Protein is a homodimeric cytokine predominantly expressed by T-lymphocytes and NK cells, and it plays a crucial role in the survival, differentiation, and chemotaxis of eosinophils. Additionally, IL-5 Protein acts on both activated and resting B-cells, inducing immunoglobulin production, growth, and differentiation. Its biological effects are mediated through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB, leading to the activation of various kinases such as LYN, SYK, and JAK2. These activated kinases propagate signals through the RAS-MAPK and JAK-STAT5 pathways. IL-5 Protein exists as a disulfide-linked homodimer and interacts with IL5RA and CSF2RB subunits.

Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.3 ng/mL.

Human

E. coli

C-His

P05113 (I20-S134)

3567  [NCBI]

MIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

Approximately 14.09 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.