IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer Protein, Human (HEK293, His-Avi)

IL-23 alpha and IL-12 beta are the IL-23p19 and IL-12p40 subunits, respectively, composing IL-23 via heterodimerization manner. IL-23 binds to a heterodimeric receptor composed of IL-12RB1 and IL-23R, activates the Jak-Stat signaling cascade, acts on memory CD4(+) T cells preferentially^[1]. IL-23 also involves in activation of several pathways including p38 MAPK or NF-κB and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A^[2]. IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer Protein, Human is produced in HEK293 cells, with total length of 476 amino acids and a C-Terminal His- and Avi-tag.

IL-23 alpha and IL-12 beta, also known as IL23p19 and IL12p40, respectively, and composing IL-23 in a heterodimerization manner, exerts proinflammatory effects and promotes angiogenesis^[1][5].
IL-23 belongs to the IL-12 cytokine family together with IL-12 p35/p40, IL-27 EBI3/p28 and IL-35 EBI3/p35, and is produced by various immune cells such as dendritic cells and macrophages upon Toll-like receptor signaling in tissues^[3].
IL-23 has a preference expression on memory CD4(+) T cells, and activates the Jak-Stat signaling cascade. IL-23 leads to IL-23 receptor phosphorylation and forms a docking site to trigger phosphorylation signal of STAT3 and STAT4^[1].
IL-23 is a key factor perpetuating Th17 cell activation and cytokine production by binding IL-23 receptor to produce Th17 cytokines such as IL17 A, IL-17 F and IL-22^[2].
IL-23 also acts function on natural killer cells, results interferon-γ secretion increasing and enhances antibody-dependent cellular cytotoxicity^[4].
The sequence of amino acids in IL-23 alpha proteins of human shows high similarity with Cynomolgus (97.88%) and is very different from mouse (74.60%) or rat (79.37%), while the sequence of IL-12 beta proteins of human is very different from mouse (69.04%) and rat (69.04%).
IL-23 facilitates development of inflammation in numerous other models of immune pathology where IL-12 had previously been implicated, including models of arthritis,intestinal inflammation, and psoriasis^[6][7][8].

Human

HEK293

C-Avi;C-His

Q9NPF7 (R20-P189)&P29460 (I23-S328)

51561  [NCBI]&3593  [NCBI]

Approximately 20 kDa

Greater than 95% as determined by Tris-Bis PAGE

<1 EU/μg, determined by LAL method.

• [1]. Oppmann B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity. 2000 Nov;13(5):715-25.  [Content Brief]

  [2]. Parham C, et al. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R. J Immunol. 2002 Jun 1;168(11):5699-708.  [Content Brief]

  [3]. Neurath MF. IL-23 in inflammatory bowel diseases and colon cancer. Cytokine Growth Factor Rev. 2019 Feb;45:1-8.  [Content Brief]

  [4]. Teng MW, et al. IL-12 and IL-23 cytokines: from discovery to targeted therapies for immune-mediated inflammatory diseases. Nat Med. 2015 Jul;21(7):719-29.  [Content Brief]

  [5]. Li Y, et al. Regulation of Memory T Cells by Interleukin-23. Int Arch Allergy Immunol. 2016;169(3):157-62.  [Content Brief]

  [6]. Langowski JL, et al. IL-23 promotes tumour incidence and growth. Nature. 2006 Jul 27;442(7101):461-5.  [Content Brief]

  [7]. Murphy CA, et al. Divergent pro- and antiinflammatory roles for IL-23 and IL-12 in joint autoimmune inflammation. J Exp Med. 2003 Dec 15;198(12):1951-7.  [Content Brief]

  [8]. Yen D, et al. IL-23 is essential for T cell-mediated colitis and promotes inflammation via IL-17 and IL-6. J Clin Invest. 2006 May;116(5):1310-6.  [Content Brief]

  [9]. Chan JR, et al. IL-23 stimulates epidermal hyperplasia via TNF and IL-20R2-dependent mechanisms with implications for psoriasis pathogenesis. J Exp Med. 2006 Nov 27;203(12):2577-87.  [Content Brief]

  [10]. Yago T, et al. IL-23 induces human osteoclastogenesis via IL-17 in vitro, and anti-IL-23 antibody attenuates collagen-induced arthritis in rats. Arthritis Res Ther. 2007;9(5):R96.  [Content Brief]