Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi)

Siglec-10 protein is thought to be a putative adhesion molecule that mediates sialic acid-dependent cell binding, preferentially selecting α-2,3- or α-2,6-linked sialic acid. Its sialic acid recognition site may be masked by cis interactions. Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived Siglec-10 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag.

Siglec-10 protein, recognized as a putative adhesion molecule, functions in sialic-acid dependent cellular binding, displaying a preference for alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site of Siglec-10 may undergo masking due to cis interactions with sialic acids on the same cell surface. In immune responses, it appears to act as an inhibitory receptor, inducing ligand-induced tyrosine phosphorylation and recruiting cytoplasmic phosphatases via their SH2 domains, blocking signal transduction through dephosphorylation of signaling molecules. Siglec-10 is involved in the negative regulation of B-cell antigen receptor signaling, dependent on PTPN6/SHP-1. In association with CD24, it may participate in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. Siglec-10, in collaboration with CD24, regulates the immune response of natural killer (NK) cells and plays a role in the control of autoimmunity. During the initiation of adaptive immune responses by CD8-alpha(+) dendritic cells, Siglec-10 inhibits cross-presentation by impairing the formation of MHC class I-peptide complexes, implicating the recruitment of PTPN6/SHP-1 and promoting phagosomal acidification. Siglec-10 interacts with various proteins, including PTPN6/SHP-1, NCF1, CD24, HMGB1, RIGI, CBL, and PTPN11.

Measured by its binding ability in a functional ELISA. 1 μg/mL (100 μL/well) of immobilized Anti-Human Siglec10 mAb can bind Siglec-10, Human (HEK 293, Fc-Avi) with an ED₅₀ value of ≤ 1.45 ng/mL.

Human

HEK293

C-Avi;C-hFc

Q96LC7-1 (M17-T546)

89790  [NCBI]

MDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKGAPVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFEECPPPSFSWTGAALSSQGTKPTTSHFSVLSFTPRPQDHNTDLTCHVDFSRKGVSAQRTVRLRVAYAPRDLVISISRDNTPALEPQPQGNVPYLEAQKGQFLRLLCAADSQPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENRLGSQQRALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQVLSPSQPSDPGVLELPRVQVEHEGEFTCHARHPLGSQHVSLSLSVHYSPKLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQDSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCEAWNVHGAQSGSILQLPDKKGLIST

100-130 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.