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TIM-1/KIM-1/HAVCR Protein, Human (HEK293, His-Fc)

Cat. No.: HY-P73604
Data Sheet Handling Instructions Technical Support

TIM-1/KIM-1/HAVCR Protein, Mouse (HEK293, His) belongs to a family of immunoglobulin-like domain-containing transmembrane proteins is widely accepted to be the receptor for hepatitis A virus (HAV), an unusual, hepatotropic human picornavirus. TIM1 (HAVCR1) facilitates attachment of a variety of enveloped viruses, including filoviruses, alphaviruses, and flaviviruses, that display highly conserved phosphatidylserine (PtdSer) on their surface. TIM1 undergoes tyrosine phosphorylation of its C-terminal cytoplasmic tail. TIM-1 can function as a co-stimulatory molecule for T cell activation. TIM-1/KIM-1/HAVCR Protein, Human (HEK293, His-Fc) is the recombinant human-derived TIM-1/KIM-1/HAVCR protein, expressed by HEK293 , with N-hFc, C-His labeled tag. The total length of TIM-1/KIM-1/HAVCR Protein, Human (HEK293, His-Fc) is 270 a.a., with molecular weight of 120-140 kDa.

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Description

TIM-1/KIM-1/HAVCR Protein, Mouse (HEK293, His) belongs to a family of immunoglobulin-like domain-containing transmembrane proteins is widely accepted to be the receptor for hepatitis A virus (HAV), an unusual, hepatotropic human picornavirus. TIM1 (HAVCR1) facilitates attachment of a variety of enveloped viruses, including filoviruses, alphaviruses, and flaviviruses, that display highly conserved phosphatidylserine (PtdSer) on their surface. TIM1 undergoes tyrosine phosphorylation of its C-terminal cytoplasmic tail. TIM-1 can function as a co-stimulatory molecule for T cell activation[1][2][3]. TIM-1/KIM-1/HAVCR Protein, Human (HEK293, His-Fc) is the recombinant human-derived TIM-1/KIM-1/HAVCR protein, expressed by HEK293 , with N-hFc, C-His labeled tag. The total length of TIM-1/KIM-1/HAVCR Protein, Human (HEK293, His-Fc) is 270 a.a., with molecular weight of 120-140 kDa.

Background

TIM1 belongs to a family of immunoglobulin-like domain-containing transmembrane proteins that include three members in humans (human TIM1, TIM3, and TIM4) and eight members in mice (murine TIM1 to TIM8), of which the human TIM1, TIM3, and TIM4 are direct orthologs of murine TIM1, TIM3, and TIM4, respectively. These proteins are expressed on the cell surface, with their N-terminal immunoglobulin-like (IgV) and mucin domains present in the extracellular milieu and their C-terminal sequences in the cytoplasm. An important feature of all TIM proteins is a highly conserved phosphatidylserine (PtdSer)-binding pocket in the IgV domain that recognizes PtdSer on the outer membrane leaflet of apoptotic cells, facilitating their uptake by phagocytic cells[1].
TIM-1 is a type I membrane protein with an IgV domain followed by a heavily glycoslyated mucin domain, a transmembrane domain and an intracellular cytoplasmic tail with one tyrosine phosphorylation motif. TIM-1 can function as a co-stimulatory molecule for T cell activation. Cross-linking Tim-1 with antibodies, in conjunction with TCR and CD28 stimulation, enhances the proliferation of CD4+ T cells. over-expression of Tim-1 leads to NFAT/AP-1 transcriptional activation, dependent on Y276 in the cytoplasmic tail[2].

Species

Human

Source

HEK293

Tag

N-hFc;C-His

Accession

AAC39862 (S21-G290)

Gene ID
Molecular Construction
N-term
hFc
TIM-1 (S21-G290)
Accession # AAC39862
His
C-term
Synonyms
Hepatitis A virus cellular receptor 1; HAVcr-1; KIM-1; TIM-1; CD365; HAVCR1
Molecular Weight

120-140 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

TIM-1/KIM-1/HAVCR Protein, Human (HEK293, His-Fc) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
TIM-1/KIM-1/HAVCR Protein, Human (HEK293, His-Fc)
Cat. No.:
HY-P73604
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