2. Neuronal Signaling
  3. Cholinesterase (ChE)
  4. Resorufin butyrate

Resorufin butyrate is a fluorogenic substrate for triglyceride lipases and cholinesterase (Ex=570 nm, Em=580 nm).

For research use only. We do not sell to patients.

Resorufin butyrate Chemical Structure

Resorufin butyrate Chemical Structure

CAS No. : 15585-42-9

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Resorufin butyrate Related Antibodies

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Description

Resorufin butyrate is a fluorogenic substrate for triglyceride lipases and cholinesterase (Ex=570 nm, Em=580 nm)[1][2][3].

IC50 & Target

Triglyceride lipases, cholinesterase[1]
In Vitro

Resorufin butyrate (2.3 μM) displays a linear increase in fluorescence over 60 min (conducted in 384-well plates using 12 nM of bLPL enzyme and substrate in PBS, pH 7.5, 5% DMSO)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Resorufin butyrate Related Antibodies
Molecular Weight

283.28

Formula

C16H13NO4
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

580
Excitation (Ex)

570
SMILES

O=C1C=CC2=NC3=C(OC2=C1)C=C(OC(CCC)=O)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (17.65 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5301 mL 17.6504 mL 35.3008 mL
5 mM 0.7060 mL 3.5301 mL 7.0602 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

SDS (251 KB)

COA (244 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5301 mL 17.6504 mL 35.3008 mL 88.2519 mL
5 mM 0.7060 mL 3.5301 mL 7.0602 mL 17.6504 mL
10 mM 0.3530 mL 1.7650 mL 3.5301 mL 8.8252 mL
15 mM 0.2353 mL 1.1767 mL 2.3534 mL 5.8835 mL
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Resorufin butyrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Resorufin butyrate15585-42-9Cholinesterase (ChE)Lipasefluorogenic substrateInhibitorinhibitorinhibit

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