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  6. Retrorsine

Retrorsine is a naturally occurring toxic pyrrolizidine alkaloid. Retrorsine can bind with DNA and inhibits the proliferative capacity of hepatocytes. Retrorsine can be used for the research of hepatocellular injury.

For research use only. We do not sell to patients.

Retrorsine Chemical Structure

Retrorsine Chemical Structure

CAS No. : 480-54-6

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10 mg USD 160 In-stock
25 mg USD 320 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Retrorsine:

Retrorsine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Retrorsine

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Retrorsine is a naturally occurring toxic pyrrolizidine alkaloid. Retrorsine can bind with DNA and inhibits the proliferative capacity of hepatocytes. Retrorsine can be used for the research of hepatocellular injury[1][2].

In Vitro

Retrorsine (60-240 μM; 24 hours) significantly reduces HSEC-CYP3A4 cells viability and GSH levels, and increases formation of pyrrole-protein adducts[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Retrorsine Related Antibodies

Cell Viability Assay[3]

Cell Line: HSEC-CYP3A4 cells
Concentration: 60 μM, 120 μM , 240 μM
Incubation Time: 24 hours
Result: Significantly decreased cell viability.
In Vivo

Retrorsine (30 mg/kg; i.p.; twice) impairs liver regeneration in the PBL model not only by an S or G2/M phase block, but also by a block located before the G1/S transition of the cell cycle[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (180±20 g), portal branch ligation (PBL) model[4]
Dosage: 30 mg/kg
Administration: Intraperitoneal injection, twice, separated by 2-week interval
Result: Strongly impaired the liver weight gain, protein and DNA synthesis as well as induction of cell cycle related proteins in the regenerating lobes after PBL.
Molecular Weight

351.39

Formula

C18H25NO6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O[C@]1([H])CCN2[C@]1([H])C(COC([C@](CO)(O)[C@H](C)C/3)=O)=CC2)C3=C/C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (284.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8458 mL 14.2292 mL 28.4584 mL
5 mM 0.5692 mL 2.8458 mL 5.6917 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.63%

COA (259 KB) HNMR (280 KB) LCMS (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8458 mL 14.2292 mL 28.4584 mL 71.1460 mL
5 mM 0.5692 mL 2.8458 mL 5.6917 mL 14.2292 mL
10 mM 0.2846 mL 1.4229 mL 2.8458 mL 7.1146 mL
15 mM 0.1897 mL 0.9486 mL 1.8972 mL 4.7431 mL
20 mM 0.1423 mL 0.7115 mL 1.4229 mL 3.5573 mL
25 mM 0.1138 mL 0.5692 mL 1.1383 mL 2.8458 mL
30 mM 0.0949 mL 0.4743 mL 0.9486 mL 2.3715 mL
40 mM 0.0711 mL 0.3557 mL 0.7115 mL 1.7787 mL
50 mM 0.0569 mL 0.2846 mL 0.5692 mL 1.4229 mL
60 mM 0.0474 mL 0.2372 mL 0.4743 mL 1.1858 mL
80 mM 0.0356 mL 0.1779 mL 0.3557 mL 0.8893 mL
100 mM 0.0285 mL 0.1423 mL 0.2846 mL 0.7115 mL
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Retrorsine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Retrorsine480-54-6OthershepatotoxicpyrrolizidinealkaloidPAhepatocellularinjuryInhibitorinhibitorinhibit

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