2. Neuronal Signaling
  3. Cholinesterase (ChE)
  4. Rhodiosin

Rhodiosin is a double inhibitor of CYP2D6 and AChE, and can be isolated from Rhodiolis rhodiolis root. The IC50 for CYP2D6 is 0.761 μM, and the Ki is 0.769 μM. Rhodiosin has antioxidant and neuroprotective activity and can regulate HIF-1α signaling pathway to protect the central nervous system

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Rhodiosin Chemical Structure

Rhodiosin Chemical Structure

CAS No. : 86831-54-1

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Based on 1 publication(s) in Google Scholar

Other Forms of Rhodiosin:

Rhodiosin Related Antibodies

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Description

Rhodiosin is a double inhibitor of CYP2D6 and AChE, and can be isolated from Rhodiolis rhodiolis root. The IC50 for CYP2D6 is 0.761 μM, and the Ki is 0.769 μM. Rhodiosin has antioxidant and neuroprotective activity and can regulate HIF-1α signaling pathway to protect the central nervous system[1][2][3][4]

IC50 & Target

AChE

 

In Vitro

Rhodiosin (10 μM, 24 h) regulates the protective effect of the central nervous system by affecting the HIF-1α signaling pathway [3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rhodiosin Related Antibodies

Western Blot Analysis [3]

Cell Line: BV-2 and PC-12
Concentration: 10 μM
Incubation Time: 24 h
Result: Reduced HIF-1α degradation in BV-2 and PC-12 cells under normoxia, and enhanced the expression of HIF-1α protein in PC-12 cells under hypoxic conditions.
In Vivo

Rhodiosin (100 mg/kg, Oral gavage (p.o.), once a day for 10 days) can reduce MDA content in irradiated C57BL mice models and reduce liver damage induced by radiation[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Irradiation C57BL mice model[4]
Dosage: 100 mg/kg, once a day for 10 days
Administration: Oral gavage (p.o.)
Result: Reduced MDA content in the liver induced by irradiation.
Molecular Weight

610.52

Formula

C27H30O16
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

OC1=C2C(C(C(O)=C(C3=CC=C(O)C=C3)O2)=O)=C(O)C=C1O[C@H]4[C@@H]([C@@H]([C@@H](O)[C@H](C)O4)O[C@]5([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]5O)CO)O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (54.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6379 mL 8.1897 mL 16.3795 mL
5 mM 0.3276 mL 1.6379 mL 3.2759 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.36%

SDS (393 KB)

COA (250 KB) HNMR (243 KB) LCMS (265 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6379 mL 8.1897 mL 16.3795 mL 40.9487 mL
5 mM 0.3276 mL 1.6379 mL 3.2759 mL 8.1897 mL
10 mM 0.1638 mL 0.8190 mL 1.6379 mL 4.0949 mL
15 mM 0.1092 mL 0.5460 mL 1.0920 mL 2.7299 mL
20 mM 0.0819 mL 0.4095 mL 0.8190 mL 2.0474 mL
25 mM 0.0655 mL 0.3276 mL 0.6552 mL 1.6379 mL
30 mM 0.0546 mL 0.2730 mL 0.5460 mL 1.3650 mL
40 mM 0.0409 mL 0.2047 mL 0.4095 mL 1.0237 mL
50 mM 0.0328 mL 0.1638 mL 0.3276 mL 0.8190 mL
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Rhodiosin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rhodiosin86831-54-1Cholinesterase (ChE)AChEInhibitor,CYP2D6HIF-1αInhibitorinhibitorinhibit

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