RIP1 kinase inhibitor 7

Cat. No.: HY-153437: FAQs
Data Sheet Handling Instructions

Molarity Calculator

RIP1 kinase inhibitor 7 (compound 41) is a potent receptor interacting protein 1 kinase (RIP1) Inhibitor with an IC₅₀ of <100 nM for human RIP1. RIP1 kinase inhibitor 7 has an EC₅₀ of 1-100 nM in the cell necrosis assay.

For research use only. We do not sell to patients.

RIP1 kinase inhibitor 7 Chemical Structure

CAS No. : 2428401-23-2

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RIPK1 RIPK2 RIPK3

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

hRIP1

<100 nM μM (IC₅₀)

Molecular Weight

337.39

Formula

C₂₀H₂₀FN₃O

CAS No.

2428401-23-2

SMILES

O=C(N1CC(C1)CC2=CC(F)=CC=C2)N3N=CCC3C4=CC=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 66.67 mg/mL (197.61 mM; ultrasonic and warming and heat to 160°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9639 mL	14.8196 mL	29.6393 mL
5 mM	0.5928 mL	2.9639 mL	5.9279 mL
10 mM	0.2964 mL	1.4820 mL	2.9639 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (14.82 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (14.82 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 5 mg/mL (14.82 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Yaning SU, et al. Cyclic Ureas. WO2020103884A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9639 mL	14.8196 mL	29.6393 mL	74.0982 mL
	5 mM	0.5928 mL	2.9639 mL	5.9279 mL	14.8196 mL
	10 mM	0.2964 mL	1.4820 mL	2.9639 mL	7.4098 mL
	15 mM	0.1976 mL	0.9880 mL	1.9760 mL	4.9399 mL
	20 mM	0.1482 mL	0.7410 mL	1.4820 mL	3.7049 mL
	25 mM	0.1186 mL	0.5928 mL	1.1856 mL	2.9639 mL
	30 mM	0.0988 mL	0.4940 mL	0.9880 mL	2.4699 mL
	40 mM	0.0741 mL	0.3705 mL	0.7410 mL	1.8525 mL
	50 mM	0.0593 mL	0.2964 mL	0.5928 mL	1.4820 mL
	60 mM	0.0494 mL	0.2470 mL	0.4940 mL	1.2350 mL
	80 mM	0.0370 mL	0.1852 mL	0.3705 mL	0.9262 mL
	100 mM	0.0296 mL	0.1482 mL	0.2964 mL	0.7410 mL