2. Cell Cycle/DNA Damage Apoptosis
  3. Eukaryotic Initiation Factor (eIF) Apoptosis
  4. Rohinitib

Rohinitib is a potent and specific eIF4A inhibitor. Rohinitib induces cell apoptosis of acute myeloid leukemia (AML) cell lines and reduces the leukemia burden of AML xenograft model. Rohinitib can be used for the research of AML.

For research use only. We do not sell to patients.

Rohinitib Chemical Structure

Rohinitib Chemical Structure

CAS No. : 1139253-73-8

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5 mg USD 450 In-stock
10 mg USD 800 In-stock
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Based on 1 publication(s) in Google Scholar

Rohinitib Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Rohinitib is a potent and specific eIF4A inhibitor. Rohinitib induces cell apoptosis of acute myeloid leukemia (AML) cell lines and reduces the leukemia burden of AML xenograft model. Rohinitib can be used for the research of AML[1].

In Vitro

Rohinitib (6.25-50 nM; 72 h) induces cell apoptosis of AML cell lines and FLT3-ITD-positive AML cell lines[1].
Primary AML cells is more sensitive to Rohinitib (25 nM; 72 h) than normal bone marrow (BM) and FLT3-ITD-positive cells is more sensitive to Rohinitib than FLT3 wild-type AML cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rohinitib Related Antibodies

Apoptosis Analysis[1]

Cell Line: AML cell lines
Concentration: 6.25, 12.5, 25 and 50 nM
Incubation Time: 72 h
Result: Dose-dependently induced apoptosis of MOLM-13, MOLM-14, MV4;11, OCI-AML3, THP-1, HL-60, Kasumi-1 and NB4 cell lines. And significantly induced cell apoptosis of FLT3-ITD, FLT3-ITD-expressing murine Ba/F3 and human OCI-AML3 cells.
In Vivo

Rohinitib (0.75 and 1.0 mg/kg; s.c. once daily for 5 consecutive days until mice get moribund) shows anti-AML effects in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female NSG mice with AML xenografts generated by intravenous injections of MOLM-13 cells[1]
Dosage: 0.75 and 1.0 mg/kg
Administration: Subcutaneous injection; 0.75 and 1.0 mg/kg once daily 5 days a week until mice get moribund
Result: Significantly reduced the leukemia burden, circulating and BM leukemic human CD45+ cells. Dose-dependently prolonged the survival rate of mice.
Molecular Weight

521.56

Formula

C29H31NO8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

COC1=CC=C([C@@]23OC4=CC(OC)=CC(OC)=C4[C@]2(O)[C@@H]([C@@H]([C@H]3C5=CC=CC=C5)C(N(OC)C)=O)O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (383.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9173 mL 9.5866 mL 19.1732 mL
5 mM 0.3835 mL 1.9173 mL 3.8346 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (9.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (9.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9173 mL 9.5866 mL 19.1732 mL 47.9331 mL
5 mM 0.3835 mL 1.9173 mL 3.8346 mL 9.5866 mL
10 mM 0.1917 mL 0.9587 mL 1.9173 mL 4.7933 mL
15 mM 0.1278 mL 0.6391 mL 1.2782 mL 3.1955 mL
20 mM 0.0959 mL 0.4793 mL 0.9587 mL 2.3967 mL
25 mM 0.0767 mL 0.3835 mL 0.7669 mL 1.9173 mL
30 mM 0.0639 mL 0.3196 mL 0.6391 mL 1.5978 mL
40 mM 0.0479 mL 0.2397 mL 0.4793 mL 1.1983 mL
50 mM 0.0383 mL 0.1917 mL 0.3835 mL 0.9587 mL
60 mM 0.0320 mL 0.1598 mL 0.3196 mL 0.7989 mL
80 mM 0.0240 mL 0.1198 mL 0.2397 mL 0.5992 mL
100 mM 0.0192 mL 0.0959 mL 0.1917 mL 0.4793 mL
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Rohinitib Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rohinitib1139253-73-8Eukaryotic Initiation Factor (eIF)ApoptosisAMLapoptosismiceFLT3MOLM-13Inhibitorinhibitorinhibit

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