1. Autophagy Apoptosis Protein Tyrosine Kinase/RTK
  2. Autophagy Apoptosis ROS Kinase
  3. Salvigenin

Salvigenin  (Synonyms: Psathyrotin)

Cat. No.: HY-N1318 Purity: 99.93%
COA Handling Instructions

Salvigenin is a natural polyphenolic compound, with neuroprotective effect. Salvigenin has antitumor cytotoxic and immunomodulatory properties. Salvigenin inhibits H2O2-induced cell apoptosis.

For research use only. We do not sell to patients.

Salvigenin Chemical Structure

Salvigenin Chemical Structure

CAS No. : 19103-54-9

Size Price Stock Quantity
1 mg USD 78 In-stock
5 mg USD 195 In-stock
10 mg USD 320 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Salvigenin:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Salvigenin is a natural polyphenolic compound, with neuroprotective effect. Salvigenin has antitumor cytotoxic and immunomodulatory properties. Salvigenin inhibits H2O2-induced cell apoptosis[1][2].

In Vitro

Salvigenin (0-100 μM, 3 h) protects SH-SY5Y cells against H2O2-induced cell death[1].
Salvigenin (0-100 μM, 2 h) significantly reduces ROS generation, increases the glutathione level, declines the extent of cell apoptosis and increases autophagy in H2O2-exposed SH-SY5Y cells[1].
Note: Please sterilize the working solution by using a 0.22 μm sterile filter membrane before cell experiments.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: SY5Y cells exposed to H2O2 (300 μM)
Concentration: 10, 25, 50, and 100 μM
Incubation Time: 3 h
Result: Protected SH-SY5Y cells (best in 25 and 50μM) against H2O2-induced cell death.

Apoptosis Analysis[1]

Cell Line: SY5Y cells exposed to H2O2 (300 μM)
Concentration: 10, 25, 50, and 100 μM
Incubation Time: 2 h
Result: Significantly declined the extent of cell apoptosis induced by H2O2.

Western Blot Analysis[1]

Cell Line: SY5Y cells exposed to H2O2 (300 μM)
Concentration: 10, 25, 50, and 100 μM
Incubation Time: 2 h
Result: Reduced cleaved caspase-3 and Bax/Bcl-2 ratio. Reduced caspase-12 and calpain levels. Reduced the level of Atg7, Atg12, and LC3-II/ LC3-I.
In Vivo

Salvigenin (0-9.68 μg/mouse/day; i.p.; 4 or 12 days) shows antitumor and immunomodulatory effects on tumor bearing mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MCF-7 cell bearing inbred female Balb/c mice, aged from six to eight weeks[2]
Dosage: 3.65, 5.85 and 9.68 μg/mouse/day for four days or 12 days
Administration: Intraperitoneal injection
Result: Showed a significant increase in DTH response in a dose-dependent manner. Significantly decreased the rate of tumor growth, increased lymphocyte proliferation index, increased the level of IFN-γ and decreased IL-4 production. Exhibited a significant decrease of the splenic CD4+CD25+Foxp3+ T lymphocytes.
Molecular Weight

328.32

Formula

C18H16O6

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C1C=C(C2=CC=C(OC)C=C2)OC3=CC(OC)=C(OC)C(O)=C13

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (50.77 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0458 mL 15.2290 mL 30.4581 mL
5 mM 0.6092 mL 3.0458 mL 6.0916 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2 mg/mL (6.09 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.93%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0458 mL 15.2290 mL 30.4581 mL 76.1452 mL
5 mM 0.6092 mL 3.0458 mL 6.0916 mL 15.2290 mL
10 mM 0.3046 mL 1.5229 mL 3.0458 mL 7.6145 mL
15 mM 0.2031 mL 1.0153 mL 2.0305 mL 5.0763 mL
20 mM 0.1523 mL 0.7615 mL 1.5229 mL 3.8073 mL
25 mM 0.1218 mL 0.6092 mL 1.2183 mL 3.0458 mL
30 mM 0.1015 mL 0.5076 mL 1.0153 mL 2.5382 mL
40 mM 0.0761 mL 0.3807 mL 0.7615 mL 1.9036 mL
50 mM 0.0609 mL 0.3046 mL 0.6092 mL 1.5229 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Salvigenin
Cat. No.:
HY-N1318
Quantity:
MCE Japan Authorized Agent: