SARS-CoV-2 nsp14-IN-3

Name: SARS-CoV-2 nsp14-IN-3
Brand: MedChemExpress
SKU: HY-149321
Rating: 4.5 (1 reviews)

Cat. No.: HY-149321 Purity: ≥95.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SARS-CoV-2 nsp14-IN-3 (4975) is an inhibitor of the SARS-CoV-2 Nsp14 N7-Methyltransferase (IC₅₀: 3.5 μM).

For research use only. We do not sell to patients.

SARS-CoV-2 nsp14-IN-3 Chemical Structure

CAS No. : 2920574-16-7

Size	Price	Stock	Quantity
5 mg	USD 345	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 555	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1780	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2850	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

SARS-CoV-2 nsp14-IN-3 (4975) is an inhibitor of the SARS-CoV-2 Nsp14 N7-Methyltransferase (IC₅₀: 3.5 μM)^[1].

Molecular Weight

343.40

Formula

C₁₇H₁₇N₃O₃S

CAS No.

2920574-16-7

Appearance

Solid

Color

Light yellow to light brown

SMILES

O=C1NCCN1C2=CC=CC(NC(C3=CC(C)=C(C=O)S3)=O)=C2C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (291.21 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9121 mL	14.5603 mL	29.1206 mL
5 mM	0.5824 mL	2.9121 mL	5.8241 mL
10 mM	0.2912 mL	1.4560 mL	2.9121 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (14.56 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (14.56 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 5 mg/mL (14.56 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥95.0%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (248 KB) HNMR (142 KB) LCMS (256 KB)

Handling Instructions (2659 KB)

References

[1]. Singh I, et al. Structure-Based Discovery of Inhibitors of the SARS-CoV-2 Nsp14 N7-Methyltransferase. J Med Chem. 2023 Jun 22;66(12):7785-7803. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9121 mL	14.5603 mL	29.1206 mL	72.8014 mL
	5 mM	0.5824 mL	2.9121 mL	5.8241 mL	14.5603 mL
	10 mM	0.2912 mL	1.4560 mL	2.9121 mL	7.2801 mL
	15 mM	0.1941 mL	0.9707 mL	1.9414 mL	4.8534 mL
	20 mM	0.1456 mL	0.7280 mL	1.4560 mL	3.6401 mL
	25 mM	0.1165 mL	0.5824 mL	1.1648 mL	2.9121 mL
	30 mM	0.0971 mL	0.4853 mL	0.9707 mL	2.4267 mL
	40 mM	0.0728 mL	0.3640 mL	0.7280 mL	1.8200 mL
	50 mM	0.0582 mL	0.2912 mL	0.5824 mL	1.4560 mL
	60 mM	0.0485 mL	0.2427 mL	0.4853 mL	1.2134 mL
	80 mM	0.0364 mL	0.1820 mL	0.3640 mL	0.9100 mL
	100 mM	0.0291 mL	0.1456 mL	0.2912 mL	0.7280 mL