2. Neuronal Signaling
  3. Monoamine Oxidase
  4. Semicarbazide hydrochloride

Semicarbazide hydrochloride  (Synonyms: Aminourea hydrochloride; Hydrazinecarboxamide hydrochloride)

Cat. No.: HY-Y0470 Purity: ≥98.0%
SDS
COA
Handling Instructions Technical Support

Semicarbazide hydrochloride is an orally active urea derivative. Semicarbazide hydrochloride binds to copper or iron in cells. Semicarbazide hydrochloride inhibits the activity of soluble semicarbazide sensitive amine oxidase (SSAO). Semicarbazide hydrochloride damages cartilage, blood vessels, ovaries, testicles, and thyroid follicles.

For research use only. We do not sell to patients.

Semicarbazide hydrochloride Chemical Structure

Semicarbazide hydrochloride Chemical Structure

CAS No. : 563-41-7

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Semicarbazide hydrochloride:

Semicarbazide hydrochloride Related Antibodies

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Description

Semicarbazide hydrochloride is an orally active urea derivative. Semicarbazide hydrochloride binds to copper or iron in cells. Semicarbazide hydrochloride inhibits the activity of soluble semicarbazide sensitive amine oxidase (SSAO). Semicarbazide hydrochloride damages cartilage, blood vessels, ovaries, testicles, and thyroid follicles[1][2][3][4][5][6][7][8][9].

In Vitro

Semicarbazide (1 mM) hydrochloride inhibits the activity of soluble SSAO in vitro[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Semicarbazide hydrochloride Related Antibodies
In Vivo

Semicarbazide hydrochloride (10-250 ppm in diet; p.o.; daily; 52 weeks for chronic toxicity study and 104 weeks for carcinogenicity study) causes enlargement of knee joints, reduces body weight, and histopathological changes in bone, cartilage and aorta, but shows no carcinogenicity in rats[3].
Semicarbazide hydrochloride (500-1,000 ppm in diet; p.o.; daily; 4 weeks) causes more severe osteochondral and vascular lesions in 3-week-old female SD rats compared to 20-week-old female SD rats[4].
Semicarbazide (40 mg/kg body weight; p.o.; daily; 4 weeks) hydrochloride causes distortion and degeneration of thyroid follicles in juvenile male albino rats, and most of the follicles appear normal after 4-week recovery[5].
Semicarbazide hydrochloride (40 mg/kg body weight; p.o.; daily; 4 weeks) causes a significant drop in body weight, GnRH, LH, FSH and E2 hormone levels, and histopathological alterations in ovarian tissues of adult female albino rats[6].
Semicarbazide (40 mg/kg; p.o.; daily; 4 weeks) hydrochloride causes significant structural changes in juvenile seminiferous tubules of rat testis, including vacuolated spermatogenic and Sertoli cells, discontinuity of germinal epithelium and thickened basement membrane[7].
Semicarbazide (40-140 mg/kg; p.o.; daily; 28 days) hydrochloride causes mortality at high and mid-dose levels, decreased body weight gain in male rats even at the lowest dose, lack of mineralization in cartilage at all dose levels, and histological alterations in multiple organs in juvenile Sprague-Dawley rats[8].
Semicarbazide (7.5-30.0 mg/kg, i.g., 28 days) hydrochloride causes its accumulation and affects oxidative stress as well as hepatic metabolic enzyme activity in rats[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

111.53

Formula

CH6ClN3O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NNC(N)=O.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (896.62 mM; Need ultrasonic)

DMSO : 100 mg/mL (896.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.9662 mL 44.8310 mL 89.6620 mL
5 mM 1.7932 mL 8.9662 mL 17.9324 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (22.42 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (22.42 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (448.31 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (806 KB)

COA (246 KB) HNMR (118 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 8.9662 mL 44.8310 mL 89.6620 mL 224.1549 mL
5 mM 1.7932 mL 8.9662 mL 17.9324 mL 44.8310 mL
10 mM 0.8966 mL 4.4831 mL 8.9662 mL 22.4155 mL
15 mM 0.5977 mL 2.9887 mL 5.9775 mL 14.9437 mL
20 mM 0.4483 mL 2.2415 mL 4.4831 mL 11.2077 mL
25 mM 0.3586 mL 1.7932 mL 3.5865 mL 8.9662 mL
30 mM 0.2989 mL 1.4944 mL 2.9887 mL 7.4718 mL
40 mM 0.2242 mL 1.1208 mL 2.2415 mL 5.6039 mL
50 mM 0.1793 mL 0.8966 mL 1.7932 mL 4.4831 mL
60 mM 0.1494 mL 0.7472 mL 1.4944 mL 3.7359 mL
80 mM 0.1121 mL 0.5604 mL 1.1208 mL 2.8019 mL
100 mM 0.0897 mL 0.4483 mL 0.8966 mL 2.2415 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Semicarbazide hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Semicarbazide563-41-7AminoureaHydrazinecarboxamideMonoamine OxidaseMAOSD rats，ovarianInhibitorinhibitorinhibit

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