SMAP-2 (Synonyms: DT-1154)

Name: SMAP-2
Brand: MedChemExpress
SKU: HY-120272
Rating: 4.5 (1 reviews)

Cat. No.: HY-120272 Purity: 99.63%: FAQs
Data Sheet SDS COA Handling Instructions

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SMAP-2 (DT-1154) is an orally active protein phosphatase 2A (PP2A) activator, with anti-cancer activity.

For research use only. We do not sell to patients.

SMAP-2 Chemical Structure

CAS No. : 1809068-70-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 644	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 644	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 890	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2850	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4560	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

SMAP-2 (DT-1154) is an orally active protein phosphatase 2A (PP2A) activator, with anti-cancer activity^[1]^[2].

IC₅₀ & Target

PP2A^[1]

In Vitro

SMAP-2 reduces cell viability of pancreatic ductal adenocarcinoma (PDA) cell lines in a dose-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SMAP-2 significantly reduces abdominal aortic aneurysms (AAA) incidence and aortic dilation in pro-AAA apolipoprotein E-null (ApoE-/-) mice^[2].
SMAP-2 (15 mg/kg; i.g.; daily; 6 days a week; for approximately 14 days) has anti-tumor activity in pancreatic cancer mice model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NSG mice, with PANC89 cells xenograft^[2]
Dosage:	15 mg/kg
Administration:	Oral gavage, daily, 6 days a week, for approximately 14 days
Result:	Decreased tumor growth and tumor weight.

Molecular Weight

532.57

Formula

C₂₇H₂₇F₃N₂O₄S

CAS No.

1809068-70-9

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(OC(F)(F)F)C=C1)(N[C@@H]2[C@@H](O)[C@H](N3C4=CC=CC=C4CCC5=CC=CC=C53)CCC2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 300 mg/mL (563.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8777 mL	9.3884 mL	18.7769 mL
5 mM	0.3755 mL	1.8777 mL	3.7554 mL
10 mM	0.1878 mL	0.9388 mL	1.8777 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 7.5 mg/mL (14.08 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 7.5 mg/mL (14.08 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.63%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (255 KB) LCMS (276 KB)

Handling Instructions (2659 KB)

References

[1]. Chao Zhang, et al. Abstract 13927: Allosteric Activation of PP2A Inhibits Experimental Abdominal Aortic Aneurysm. 2018. Circulation. 2016;134:A13927.

[2]. Brittany L Allen-Petersen, et al. Activation of PP2A and Inhibition of mTOR Synergistically Reduce MYC Signaling and Decrease Tumor Growth in Pancreatic Ductal Adenocarcinoma. Cancer Res. 2019 Jan 1;79(1):209-219. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8777 mL	9.3884 mL	18.7769 mL	46.9422 mL
	5 mM	0.3755 mL	1.8777 mL	3.7554 mL	9.3884 mL
	10 mM	0.1878 mL	0.9388 mL	1.8777 mL	4.6942 mL
	15 mM	0.1252 mL	0.6259 mL	1.2518 mL	3.1295 mL
	20 mM	0.0939 mL	0.4694 mL	0.9388 mL	2.3471 mL
	25 mM	0.0751 mL	0.3755 mL	0.7511 mL	1.8777 mL
	30 mM	0.0626 mL	0.3129 mL	0.6259 mL	1.5647 mL
	40 mM	0.0469 mL	0.2347 mL	0.4694 mL	1.1736 mL
	50 mM	0.0376 mL	0.1878 mL	0.3755 mL	0.9388 mL
	60 mM	0.0313 mL	0.1565 mL	0.3129 mL	0.7824 mL
	80 mM	0.0235 mL	0.1174 mL	0.2347 mL	0.5868 mL
	100 mM	0.0188 mL	0.0939 mL	0.1878 mL	0.4694 mL

SMAP-2 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SMAP-2 (Synonyms: DT-1154)

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SMAP-2 Related Antibodies

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SMAP-2 Related Antibodies

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Complete Stock Solution Preparation Table

SMAP-2 Related Classifications

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