Soyasapogenol B

Name: Soyasapogenol B
Brand: MedChemExpress
SKU: HY-N6074
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6074 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

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Soyasapogenol B is a component of soy that has oral activity. Soyasapogenol B promotes autophagy and apoptosis. Soyasapogenol B has anti-inflammatory, antioxidant and antitumor activities.

For research use only. We do not sell to patients.

Soyasapogenol B Chemical Structure

CAS No. : 595-15-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 192	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 192	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Soyasapogenol B is a component of soy that has oral activity. Soyasapogenol B promotes autophagy and apoptosis. Soyasapogenol B has anti-inflammatory, antioxidant and antitumor activities^[1]^[2]^[3].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	2.104 μM Compound: 11	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
DU-145	IC₅₀	10.93 μM Compound: 11	Cytotoxicity against human DU145 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human DU145 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
HEK293	IC₅₀	123.7 μM Compound: 11	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
HeLa	IC₅₀	3.491 μM Compound: 11	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
MCF7	IC₅₀	2.328 μM Compound: 11	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
RAW264.7	IC₅₀	> 100 μM Compound: 3	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of HMGB1-induced NO production pretreated with compounds for 15 mins followed by HMGB1 stimulation measured after 24 hrs by Griess reagent based assay Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of HMGB1-induced NO production pretreated with compounds for 15 mins followed by HMGB1 stimulation measured after 24 hrs by Griess reagent based assay	[PMID: 34800877]

In Vitro

Soyasapogenol B (5-20 μM, 48 h) can reduce tumor cell viability and promote apoptosis and autophagy of colorectal cancer cells through endoplasmic reticulum stress^[1].
Sovasapogenol B (1-10 μM, 48 h) shows anti-growth and anti-metastasis activity in clear cell renal cell carcinoma (ccRCC)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HCT116, SW480
Concentration:	5, 10, 20 μM
Incubation Time:	24, 48 h
Result:	Decreased the viability of tumor cells.

Western Blot Analysis^[1]

Cell Line:	HCT116, SW480
Concentration:	10, 20 μM
Incubation Time:	48 h
Result:	Increased the level of caspase-3 and PARP. Decreased the expression of Bcl-2 and increased the expression of Bax. Increased the expression of LC3-II/LC3-I, Beclin 1 and Atg5, decreased the expression of p62.

Apoptosis Analysis^[1]

Cell Line:	HCT116, SW480
Concentration:	10, 20 μM
Incubation Time:	48 h
Result:	Increased the number of TUNEL positive cells. Increased the apoptotic percentage in HCT116 cells by more than 4 folds and 9 folds respectively, elevated to over 10% and 20% in SW480 cells.

In Vivo

Soyasapogenol B (25 or 50 mg/kg/day, intraperitoneal injection) can inhibit tumor growth, and promote apoptosis and autophagy in a mouse tumor model of colorectal cancer^[1].
Soyasapogenol B (10 mg/kg, oral) alleviates memory impairment induced by lipopolysaccharide (HY-D1056) in mice by regulating the expression of BDNF mediated by NF-κB^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xenograft murine models^[1]
Dosage:	25 or 50 mg/kg
Administration:	i.p.
Result:	Increased the apoptotic cell death dose-dependently. Increased the expression of cleaved caspase-3, Bax, CHOP, GRP78 and LC3BII. Decreased the expression of Bcl-2 and Ki-67.

Animal Model:	Lipopolysaccharide-induced memory impairment in mice^[3]
Dosage:	10 mg/kg
Administration:	p.o.
Result:	Increased LPS-suppressed BDNF expression and inhibited NF-κB activation and TNF-α expression.

Molecular Weight

458.72

Formula

C₃₀H₅₀O₃

CAS No.

595-15-3

Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@]3(C([C@@]4([H])[C@](C)([C@@H](CC(C)(C)C4)O)CC3)=CC[C@]1([H])[C@@]5([C@@]([C@](C)([C@@H](O)CC5)CO)([H])CC2)C)C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (72.66 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1800 mL	10.8999 mL	21.7998 mL
5 mM	0.4360 mL	2.1800 mL	4.3600 mL
10 mM	0.2180 mL	1.0900 mL	2.1800 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (250 KB) HNMR (256 KB) RP-HPLC (242 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Wang L, et al. Endoplasmic reticulum stress triggered by Soyasapogenol B promotes apoptosis and autophagy in colorectal cancer. Life Sci. 2019 Feb 1;218:16-24. [Content Brief]

[2]. Wang L, et al. Soyasapogenol B exhibits anti-growth and anti-metastatic activities in clear cell renal cell carcinoma. Naunyn Schmiedebergs Arch Pharmacol. 2019 May;392(5):551-563. [Content Brief]

[3]. Lee HJ, et al. Soyasapogenol B and Genistein Attenuate Lipopolysaccharide-Induced Memory Impairment in Mice by the Modulation of NF-κB-Mediated BDNF Expression. J Agric Food Chem. 2017 Aug 16;65(32):6877-6885. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1800 mL	10.8999 mL	21.7998 mL	54.4995 mL
	5 mM	0.4360 mL	2.1800 mL	4.3600 mL	10.8999 mL
	10 mM	0.2180 mL	1.0900 mL	2.1800 mL	5.4499 mL
	15 mM	0.1453 mL	0.7267 mL	1.4533 mL	3.6333 mL
	20 mM	0.1090 mL	0.5450 mL	1.0900 mL	2.7250 mL
	25 mM	0.0872 mL	0.4360 mL	0.8720 mL	2.1800 mL
	30 mM	0.0727 mL	0.3633 mL	0.7267 mL	1.8166 mL
	40 mM	0.0545 mL	0.2725 mL	0.5450 mL	1.3625 mL
	50 mM	0.0436 mL	0.2180 mL	0.4360 mL	1.0900 mL
	60 mM	0.0363 mL	0.1817 mL	0.3633 mL	0.9083 mL

Soyasapogenol B Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Soyasapogenol B595-15-3AutophagyApoptosisAnti-inflammatoryAntitumorHCT116SW480ccRCCLPS induced memory impairmentInhibitorinhibitorinhibit

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