2. PI3K/Akt/mTOR Stem Cell/Wnt MAPK/ERK Pathway Metabolic Enzyme/Protease
  3. Akt ERK E1/E2/E3 Enzyme
  4. SPOP-IN-1

SPOP-IN-1 is a selective SPOP E3 ubiquitin ligase inhibitor. SPOP-IN-1 leads to the accumulation of tumor suppressors PTEN and DUSP7 and decreased levels of phosphorylated AKT and ERK in clear-cell renal cell carcinoma.

For research use only. We do not sell to patients.

SPOP-IN-1 Chemical Structure

SPOP-IN-1 Chemical Structure

CAS No. : 2136270-56-7

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Based on 1 publication(s) in Google Scholar

SPOP-IN-1 Related Antibodies

Top Publications Citing Use of Products

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

View All ERK Isoform Specific Products:

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ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

SPOP-IN-1 is a selective SPOP E3 ubiquitin ligase inhibitor. SPOP-IN-1 leads to the accumulation of tumor suppressors PTEN and DUSP7 and decreased levels of phosphorylated AKT and ERK in clear-cell renal cell carcinoma[1].

Cellular Effect
Cell Line Type Value Description References
A498 IC50
2.1 μM
Compound: 6lc
Antiproliferative activity against human A498 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Antiproliferative activity against human A498 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 32297747]
A549 IC50
16.2 μM
Compound: 6lc
Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 32297747]
HK-2 IC50
> 30 μM
Compound: 6lc
Cytotoxicity against human HK2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human HK2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 32297747]
Hs 766 IC50
14.5 μM
Compound: 6lc
Cytotoxicity against human Hs766T cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human Hs766T cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 32297747]
NCI-H1299 IC50
10.7 μM
Compound: 6lc
Cytotoxicity against human H1299 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human H1299 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 32297747]
OS-RC-2 IC50
3.5 μM
Compound: 6lc
Antiproliferative activity against human OS-RC2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Antiproliferative activity against human OS-RC2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 32297747]
U-251 IC50
10.2 μM
Compound: 6lc
Cytotoxicity against human U251 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human U251 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 32297747]
Molecular Weight

505.64

Formula

C26H31N7O2S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CC2=C(N(CCC3=CC=CS3)C1=N)N=C4C(C)=CC=CN4C2=O)NCCN5CCN(CC5)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (9.89 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9777 mL 9.8885 mL 19.7769 mL
5 mM 0.3955 mL 1.9777 mL 3.9554 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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This equation is commonly abbreviated as: C1V1 = C2V2

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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.71%

SDS (251 KB)

COA (249 KB) HNMR (268 KB) LCMS (98 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9777 mL 9.8885 mL 19.7769 mL 49.4423 mL
5 mM 0.3955 mL 1.9777 mL 3.9554 mL 9.8885 mL
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SPOP-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SPOP-IN-12136270-56-7AktERKE1/E2/E3 EnzymePKBProtein kinase BExtracellular signal regulated kinasesE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseSPOP E3 ubiquitin ligaseInhibitorinhibitorinhibit

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