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  4. SPOP-IN-6b dihydrochloride

SPOP-IN-6b dihydrochloride is a potent speckle-type POZ protein (SPOP) inhibitor with an IC50 of 3.58 μM.

For research use only. We do not sell to patients.

SPOP-IN-6b dihydrochloride Chemical Structure

SPOP-IN-6b dihydrochloride Chemical Structure

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5 mg USD 250 In-stock
10 mg USD 420 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of SPOP-IN-6b dihydrochloride:

SPOP-IN-6b dihydrochloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE SPOP-IN-6b dihydrochloride

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SPOP-IN-6b dihydrochloride is a potent speckle-type POZ protein (SPOP) inhibitor with an IC50 of 3.58 μM[1].

In Vitro

SPOP-IN-6b dihydrochloride shows IC50s of 2-10.2 μM for A498, Caki-2, Ketr-3, 769-P, 0S-RC-2, and 786-0 cells[1].
SPOP-IN-6b (0.1-3.0 μM; 10 hours) dihydrochloride can suppress SPOP activity, and then increase suppression cancer substrate protein PTEN and DUSP7 content[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SPOP-IN-6b dihydrochloride Related Antibodies
In Vivo

SPOP-IN-6b (40-80 mg/kg; intraperitoneal injection; daily; for 25 days; nude mice) dihydrochloride can slow down the growth of tumour by suppressing SPOP[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

573.51

Formula

C28H34Cl2N6O3
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C(C(N1CCC2=CC=CC=C2)=N)=CC3=C1N=C(C(C)=CC=C4)N4C3=O)NCCCN5CCOCC5.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 41.67 mg/mL (72.66 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7436 mL 8.7182 mL 17.4365 mL
5 mM 0.3487 mL 1.7436 mL 3.4873 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7436 mL 8.7182 mL 17.4365 mL 43.5912 mL
5 mM 0.3487 mL 1.7436 mL 3.4873 mL 8.7182 mL
10 mM 0.1744 mL 0.8718 mL 1.7436 mL 4.3591 mL
15 mM 0.1162 mL 0.5812 mL 1.1624 mL 2.9061 mL
20 mM 0.0872 mL 0.4359 mL 0.8718 mL 2.1796 mL
25 mM 0.0697 mL 0.3487 mL 0.6975 mL 1.7436 mL
30 mM 0.0581 mL 0.2906 mL 0.5812 mL 1.4530 mL
40 mM 0.0436 mL 0.2180 mL 0.4359 mL 1.0898 mL
50 mM 0.0349 mL 0.1744 mL 0.3487 mL 0.8718 mL
60 mM 0.0291 mL 0.1453 mL 0.2906 mL 0.7265 mL
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SPOP-IN-6b dihydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SPOP-IN-6bOthersA498Caki-2tumorSPOPPTENDUSP7HK-2Inhibitorinhibitorinhibit

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