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  4. SRD5A1-IN-1

SRD5A1-IN-1 (Compound 4) is a competitive and covalent steroid 5α-reductase type 1 (SRD5A1) inhibitor with an IC50 of 1.44 µM. SRD5A1-IN-1 modulates SRD5A1 function, leading to a lower level of dihydrotestosterone (DHT) production and SRD5A1 protein suppression.

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SRD5A1-IN-1 Chemical Structure

SRD5A1-IN-1 Chemical Structure

CAS No. : 2279077-93-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 319 In-stock
Solution
10 mM * 1 mL in DMSO USD 319 In-stock
Solid
5 mg USD 290 In-stock
10 mg USD 420 In-stock
25 mg USD 680 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

SRD5A1-IN-1 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

SRD5A1-IN-1 (Compound 4) is a competitive and covalent steroid 5α-reductase type 1 (SRD5A1) inhibitor with an IC50 of 1.44 µM. SRD5A1-IN-1 modulates SRD5A1 function, leading to a lower level of dihydrotestosterone (DHT) production and SRD5A1 protein suppression[1].

IC50 & Target

IC50: 1.44 µM (SRD5A1)[1]
In Vitro

SRD5A1-IN-1 (Compound 4) (0.5-2.5 μM; 24 h) decreases SRD5A1 protein expression[1].
SRD5A1-IN-1 (0-2.5 μM; 12 h) modulates SRD5A1 via the dual actions that affect the level of SRD5A1 protein expression and the activity of the SRD5A1 enzyme[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SRD5A1-IN-1 Related Antibodies

Western Blot Analysis[1]

Cell Line: HaCaT
Concentration: 0.5, 1, and 2.5 µM
Incubation Time: 12 h and 24 h
Result: Showed a significant decrease in SRD5A1 protein expression at 1 and 2.5 µM at 24 h, whereas there were no significant changes in the level of SRD5A1 protein at 12 h.

RT-PCR[1]

Cell Line: HaCaT
Concentration: 0.5, 1, and 2.5 µM
Incubation Time: 12 h and 24 h
Result: Did not affect the mRNA expression of SRD5A1 at both incubation times.

Cell Cytotoxicity Assay[1]

Cell Line: HaCaT
Concentration: 0.2, 0.5, 1, and 2.5 µM
Incubation Time: 24 h
Result: Displayed no significant cytotoxicity (IC50: 29.99 ± 8.69 µM).
Molecular Weight

391.26

Formula

C17H11F6NO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC(C(F)(F)F)=CC(C(F)(F)F)=C1)/C=C/C2=CC(O)=C(C=C2)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (255.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5558 mL 12.7792 mL 25.5585 mL
5 mM 0.5112 mL 2.5558 mL 5.1117 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.82%

COA (246 KB) HNMR (257 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5558 mL 12.7792 mL 25.5585 mL 63.8961 mL
5 mM 0.5112 mL 2.5558 mL 5.1117 mL 12.7792 mL
10 mM 0.2556 mL 1.2779 mL 2.5558 mL 6.3896 mL
15 mM 0.1704 mL 0.8519 mL 1.7039 mL 4.2597 mL
20 mM 0.1278 mL 0.6390 mL 1.2779 mL 3.1948 mL
25 mM 0.1022 mL 0.5112 mL 1.0223 mL 2.5558 mL
30 mM 0.0852 mL 0.4260 mL 0.8519 mL 2.1299 mL
40 mM 0.0639 mL 0.3195 mL 0.6390 mL 1.5974 mL
50 mM 0.0511 mL 0.2556 mL 0.5112 mL 1.2779 mL
60 mM 0.0426 mL 0.2130 mL 0.4260 mL 1.0649 mL
80 mM 0.0319 mL 0.1597 mL 0.3195 mL 0.7987 mL
100 mM 0.0256 mL 0.1278 mL 0.2556 mL 0.6390 mL
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SRD5A1-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SRD5A1-IN-12279077-93-7Otherssteroid 5α-reductase type 1SRD5A1 androgenic alopeciaInhibitorinhibitorinhibit

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