2. Cell Cycle/DNA Damage Cytoskeleton
  3. Microtubule/Tubulin
  4. ST-401

ST-401, a microtubule-targeting agent (MTA), is a brain-penetrant microtubule (MT) assembly inhibitor. ST-401 disrupts microtubule (MT) function through gentle and reverisible reduction in MT assembly that triggers mitotic delay and cell death in interphase. ST-401 shows a potent antitumor activity.

For research use only. We do not sell to patients.

ST-401 Chemical Structure

ST-401 Chemical Structure

CAS No. : 2419942-78-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 203 In-stock
Solution
10 mM * 1 mL in DMSO USD 203 In-stock
Solid
5 mg USD 185 In-stock
10 mg USD 295 In-stock
25 mg USD 590 In-stock
50 mg USD 940 In-stock
100 mg USD 1500 In-stock
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Based on 1 publication(s) in Google Scholar

ST-401 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

ST-401, a microtubule-targeting agent (MTA), is a brain-penetrant microtubule (MT) assembly inhibitor. ST-401 disrupts microtubule (MT) function through gentle and reverisible reduction in MT assembly that triggers mitotic delay and cell death in interphase. ST-401 shows a potent antitumor activity[1].

In Vitro

ST-401 is a hybrid molecule that combines a secondary alcohol and quinoline in one structure. ST-401 competes for 89% of [3H]colchicine binding to tubulin. In a turbidity assay, ST-401 inhibits tubulin assembly with an IC50 of 1.1 µM. In a pelleting assay that measures release of free tubulin, ST-401 inhibits tubulin assembly with comparable IC50 value of 3.2 µM[1].
ST-401 exhibits similar antitumor activities in the low nanomolar range in most human cancer cell lines, including the six glioma lines (SF-268, SF-295, SF-539, SNB-19, SNB-75, and U251: IC50s 23-69 nM)[1].
ST-401 shows the antitumor activity in PD-glioma is in the low nanomolar (IC50: 24-47 nM)[1].
ST-401 (72 h) reduces overall cell viability in MGG8 and T98G cells with comparable IC50s (14 and 36 nM, respectively)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ST-401 Related Antibodies
In Vivo

ST-401 (20 mg/kg; i.p; bidaily; 5 days/week for 4 weeks) demonstrates significant antitumor activity and no over toxicity in a xenograft flank tumor mouse model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Nude mice (10-14 weeks of age) injection with COLO205 cells[1]
Dosage: 20 mg/kg
Administration: i.p; bidaily; 5 days/week for 4 weeks
Result: Resulted in significant antitumor activity.
Molecular Weight

352.43

Formula

C24H20N2O
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

OC(C1=CC=CC2=C1C=CC=N2)C3=CC4=C(C=C3)N(CC)C5=CC=CC=C54
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (283.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8374 mL 14.1872 mL 28.3744 mL
5 mM 0.5675 mL 2.8374 mL 5.6749 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (14.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (14.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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% 
+
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8374 mL 14.1872 mL 28.3744 mL 70.9361 mL
5 mM 0.5675 mL 2.8374 mL 5.6749 mL 14.1872 mL
10 mM 0.2837 mL 1.4187 mL 2.8374 mL 7.0936 mL
15 mM 0.1892 mL 0.9458 mL 1.8916 mL 4.7291 mL
20 mM 0.1419 mL 0.7094 mL 1.4187 mL 3.5468 mL
25 mM 0.1135 mL 0.5675 mL 1.1350 mL 2.8374 mL
30 mM 0.0946 mL 0.4729 mL 0.9458 mL 2.3645 mL
40 mM 0.0709 mL 0.3547 mL 0.7094 mL 1.7734 mL
50 mM 0.0567 mL 0.2837 mL 0.5675 mL 1.4187 mL
60 mM 0.0473 mL 0.2365 mL 0.4729 mL 1.1823 mL
80 mM 0.0355 mL 0.1773 mL 0.3547 mL 0.8867 mL
100 mM 0.0284 mL 0.1419 mL 0.2837 mL 0.7094 mL
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ST-401 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ST-4012419942-78-0ST401ST 401Microtubule/Tubulinmicrotubule targeting agentbrain-penetrant MTAgliomaantitumor activitybrain-penetrantInhibitorinhibitorinhibit

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