Sulfobromophthalein disodium salt (Synonyms: Bromosulfophthalein disodium salt)

Name: Sulfobromophthalein disodium salt
Brand: MedChemExpress
SKU: HY-D0217
Rating: 5.0 (3 reviews)

Cat. No.: HY-D0217 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Sulfobromophthalein (Bromosulfophthalein) disodium salt is an organic anion dye used in the study of a variety of membrane carriers expressed in animal tissues and involved in transport of agents and metabolites.

For research use only. We do not sell to patients.

Sulfobromophthalein disodium salt Chemical Structure

CAS No. : 71-67-0

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Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Sulfobromophthalein disodium salt

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Description

In Vitro

Sulfobromophthalein disodium salt is a synthetic dye introduced in 1925 as a clinical tool aiming at the assessment of the liver function^[1].
Sulfobromophthalein (BSP) is a pH indicator dye, featured by fast (17), protondependent quinoidal–phenolic tautomerism (pK = 8.5) ^[1].
Bromosulfalein has been commonly used as both a substrate and inhibitor of organic anionic transporting polypeptide 1B1 (OATP1B1), OATP1B3, OATP1A2, and OATP2B1, as well as multidrug resistance protein 2 (MDR2) ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

838.00

Formula

C₂₀H₈Br₄Na₂O₁₀S₂

CAS No.

71-67-0

Appearance

Solid

Color

White to off-white

SMILES

O=C1OC(C2=CC(S(=O)(O[Na])=O)=C(O)C=C2)(C3=CC(S(=O)(O[Na])=O)=C(O)C=C3)C4=C1C(Br)=C(Br)C(Br)=C4Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (298.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1933 mL	5.9666 mL	11.9332 mL
5 mM	0.2387 mL	1.1933 mL	2.3866 mL
10 mM	0.1193 mL	0.5967 mL	1.1933 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4.17 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 4.17 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 4.17 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (285 KB) SDS (419 KB)

COA (265 KB) HNMR (283 KB)

Handling Instructions (2659 KB)

References

[1]. Saki Izumi, et al. Substrate-dependent inhibition of organic anion transporting polypeptide 1B1: comparative analysis with prototypical probe substrates estradiol-17β-glucuronide, estrone-3-sulfate, and sulfobromophthalein. Drug Metab Dispos. 2013 Oct;41(10):1859-66. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1933 mL	5.9666 mL	11.9332 mL	29.8329 mL
	5 mM	0.2387 mL	1.1933 mL	2.3866 mL	5.9666 mL
	10 mM	0.1193 mL	0.5967 mL	1.1933 mL	2.9833 mL
	15 mM	0.0796 mL	0.3978 mL	0.7955 mL	1.9889 mL
	20 mM	0.0597 mL	0.2983 mL	0.5967 mL	1.4916 mL
	25 mM	0.0477 mL	0.2387 mL	0.4773 mL	1.1933 mL
	30 mM	0.0398 mL	0.1989 mL	0.3978 mL	0.9944 mL
	40 mM	0.0298 mL	0.1492 mL	0.2983 mL	0.7458 mL
	50 mM	0.0239 mL	0.1193 mL	0.2387 mL	0.5967 mL
	60 mM	0.0199 mL	0.0994 mL	0.1989 mL	0.4972 mL
	80 mM	0.0149 mL	0.0746 mL	0.1492 mL	0.3729 mL
	100 mM	0.0119 mL	0.0597 mL	0.1193 mL	0.2983 mL

Sulfobromophthalein disodium salt Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulfobromophthalein disodium71-67-0Bromosulfophthalein disodiumFluorescent DyeBSPOATP1BMDR2Inhibitorinhibitorinhibit

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Sulfobromophthalein disodium salt (Synonyms: Bromosulfophthalein disodium salt)

Customer Review

Sulfobromophthalein disodium salt Related Antibodies

3 Publications Citing Use of MCE Sulfobromophthalein disodium salt

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Sulfobromophthalein disodium salt Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Sulfobromophthalein disodium salt Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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