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  4. Sulfobromophthalein disodium salt

Sulfobromophthalein disodium salt  (Synonyms: Bromosulfophthalein disodium salt)

Cat. No.: HY-D0217 Purity: ≥98.0%
COA Handling Instructions

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Sulfobromophthalein (Bromosulfophthalein) disodium salt is an organic anion dye used in the study of a variety of membrane carriers expressed in animal tissues and involved in transport of agents and metabolites.

For research use only. We do not sell to patients.

Sulfobromophthalein disodium salt Chemical Structure

Sulfobromophthalein disodium salt Chemical Structure

CAS No. : 71-67-0

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500 mg USD 90 In-stock
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Based on 3 publication(s) in Google Scholar

Sulfobromophthalein disodium salt Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Sulfobromophthalein (Bromosulfophthalein) disodium salt is an organic anion dye used in the study of a variety of membrane carriers expressed in animal tissues and involved in transport of agents and metabolites[1].

In Vitro

Sulfobromophthalein disodium salt is a synthetic dye introduced in 1925 as a clinical tool aiming at the assessment of the liver function[1].
Sulfobromophthalein (BSP) is a pH indicator dye, featured by fast (17), protondependent quinoidal–phenolic tautomerism (pK = 8.5) [1].
Bromosulfalein has been commonly used as both a substrate and inhibitor of organic anionic transporting polypeptide 1B1 (OATP1B1), OATP1B3, OATP1A2, and OATP2B1, as well as multidrug resistance protein 2 (MDR2) [2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sulfobromophthalein disodium salt Related Antibodies
Molecular Weight

838.00

Formula

C20H8Br4Na2O10S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1OC(C2=CC(S(=O)(O[Na])=O)=C(O)C=C2)(C3=CC(S(=O)(O[Na])=O)=C(O)C=C3)C4=C1C(Br)=C(Br)C(Br)=C4Br
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (298.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1933 mL 5.9666 mL 11.9332 mL
5 mM 0.2387 mL 1.1933 mL 2.3866 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 4.17 mg/mL (4.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 4.17 mg/mL (4.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (265 KB) HNMR (283 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1933 mL 5.9666 mL 11.9332 mL 29.8329 mL
5 mM 0.2387 mL 1.1933 mL 2.3866 mL 5.9666 mL
10 mM 0.1193 mL 0.5967 mL 1.1933 mL 2.9833 mL
15 mM 0.0796 mL 0.3978 mL 0.7955 mL 1.9889 mL
20 mM 0.0597 mL 0.2983 mL 0.5967 mL 1.4916 mL
25 mM 0.0477 mL 0.2387 mL 0.4773 mL 1.1933 mL
30 mM 0.0398 mL 0.1989 mL 0.3978 mL 0.9944 mL
40 mM 0.0298 mL 0.1492 mL 0.2983 mL 0.7458 mL
50 mM 0.0239 mL 0.1193 mL 0.2387 mL 0.5967 mL
60 mM 0.0199 mL 0.0994 mL 0.1989 mL 0.4972 mL
80 mM 0.0149 mL 0.0746 mL 0.1492 mL 0.3729 mL
100 mM 0.0119 mL 0.0597 mL 0.1193 mL 0.2983 mL
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Sulfobromophthalein disodium salt Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulfobromophthalein disodium71-67-0Bromosulfophthalein disodiumFluorescent DyeBSPOATP1BMDR2Inhibitorinhibitorinhibit

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