Tanshindiol C

Name: Tanshindiol C
Brand: MedChemExpress
SKU: HY-122936
Rating: 4.5 (1 reviews)

Cat. No.: HY-122936 Purity: 98.47%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Tanshindiol C is a S-adenosylmethionine-competitive EZH2 (Histone Methyltransferase) inhibitor with an IC₅₀ of 0.55 μM for inhibiting the methyltransferase activity. Tanshindiol C is also an activator of both Nrf2 and Sirtuin 1 (Sirt1) in macrophages. Tanshindiol C possesses anti-cancer activity, and can be used for atherosclerosis research.

For research use only. We do not sell to patients.

Tanshindiol C Chemical Structure

CAS No. : 97465-71-9

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Methyltransferase Isoform Specific Products:

View All Isoforms

EZH1 EZH2 DOT1L SMYD2 SMYD3 SUV39H2/KMT1B EHMT2/G9a/KMT1C EHMT1/GLP/KMT1D SETDB1/KMT2G SETD2/KMT3A NSD2/MMSET/WHSC1 SETD7/KMT7 SETD8/KMT5A PRMT1 PRMT3 PRMT4 PRMT5 PRMT6 PRMT7 PRMT8

View All Sirtuin Isoform Specific Products:

View All Isoforms

Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]

EZH2

0.55 μM (IC₅₀)

SIRT1

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	4.2 μM Compound: 5	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by WST-1 assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by WST-1 assay	[PMID: 24767850]
Daudi	GI₅₀	10.6 μM Compound: 5	Cytotoxicity against human Daudi cells assessed as growth inhibition after 72 hrs by WST-1 assay Cytotoxicity against human Daudi cells assessed as growth inhibition after 72 hrs by WST-1 assay	[PMID: 24767850]
PC-3	GI₅₀	4 μM Compound: 5	Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by WST-1 assay Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by WST-1 assay	[PMID: 24767850]
Pfeiffer	GI₅₀	1.5 μM Compound: 5	Cytotoxicity against human Pfeiffer cells expressing EZH2 A667G mutant assessed as growth inhibition after 72 hrs by WST-1 assay Cytotoxicity against human Pfeiffer cells expressing EZH2 A667G mutant assessed as growth inhibition after 72 hrs by WST-1 assay	[PMID: 24767850]
T98G	GI₅₀	6 μM Compound: 5	Cytotoxicity against human T98G cells assessed as growth inhibition after 72 hrs by WST-1 assay Cytotoxicity against human T98G cells assessed as growth inhibition after 72 hrs by WST-1 assay	[PMID: 24767850]
U2932	GI₅₀	9.5 μM Compound: 5	Cytotoxicity against human U2932 cells assessed as growth inhibition after 72 hrs by WST-1 assay Cytotoxicity against human U2932 cells assessed as growth inhibition after 72 hrs by WST-1 assay	[PMID: 24767850]
U-87MG ATCC	GI₅₀	5.7 μM Compound: 5	Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by WST-1 assay Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by WST-1 assay	[PMID: 24767850]

In Vitro

Tanshindiol C (1-10 μM; for 24 h) activates Nrf2 and upregulates Prdx1 expression and mRNA levels in macrophages. Tanshindiol C protects macrophages from oxidized low-density lipoprotein (oxLDL) induced foam cell formation via activation of Prdx1/ABCA1 signaling^[1].
Tanshindiol C inhibits both wild-type and A667G mutant (K_i value of 176 nM) EZH2 activity with similar potencies^[2].
Tanshindiol C inhibits growth of the cell lines such as Pfeiffer, U-2932 and Daudi (lymphoma), PC3 (prostate cancer), T98G and U87MG (glioma), and A549 (lung cancer), with GI₅₀ values of 1.5 μM, 9.5 μM, 10.6 μM, 4 μM, 6 μM, 5.7 μM and 4.2 μM, respectively^[2].
Tanshindiol C (1-5 μM; 72 hours) induces accumulation of Pfeiffer cells in sub-G1 phase, which indicates cells in late apoptosis and necrosis^[2].
Tanshindiol C (1-3 μM; 72 hours) increases protein levels of the important apoptosis related protein markers, cleaved caspase-3, caspase-7 and poly ADP-ribose polymerase (PRAP) in the cells.Tanshindiol C significantly decreases the levels of H3K27me3 in cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	RAW264.7 cells
Concentration:	1 μM, 3 μM, 10 μM
Incubation Time:	24 h
Result:	Upregulated the Nrf2 and Prdx1 mRNA levels.

Western Blot Analysis^[1]

Cell Line:	Mouse peritoneal macrophages
Concentration:	1 μM, 3 μM, 10 μM
Incubation Time:	24 h
Result:	Activated Nrf2 and upregulated Prdx1 expression in macrophages.

Cell Cycle Analysis^[2]

Cell Line:	Pfeiffer cells
Concentration:	1 μM, 2.5 μM and 5 μM
Incubation Time:	72 hours
Result:	Induced accumulation of Pfeiffer cells in sub-G1 phase.

Western Blot Analysis^[2]

Cell Line:	Pfeiffer cells
Concentration:	1 μM, 3 μM
Incubation Time:	72 hours
Result:	The levels of H3K27me3 was significantly decreased in the cells.

Molecular Weight

312.32

Formula

C₁₈H₁₆O₅

CAS No.

97465-71-9

Appearance

Solid

Color

Brown to red

SMILES

O=C(C1=C2C=CC3=C1CC[C@@H](O)[C@@]3(O)C)C(C4=C2OC=C4C)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (80.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2018 mL	16.0092 mL	32.0184 mL
5 mM	0.6404 mL	3.2018 mL	6.4037 mL
10 mM	0.3202 mL	1.6009 mL	3.2018 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.47%

Select Batch:

Data Sheet (279 KB) SDS (251 KB)

COA (255 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Yuyu Yang, et al. Tanshindiol C inhibits oxidized low-density lipoprotein induced macrophage foam cell formation via a peroxiredoxin 1 dependent pathway. Biochim Biophys Acta Mol Basis Dis. 2018 Mar;1864(3):882-890. [Content Brief]

[2]. Jimin Woo, et al. Biological evaluation of tanshindiols as EZH2 histone methyltransferase inhibitors. Bioorg Med Chem Lett. 2014 Jun 1;24(11):2486-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2018 mL	16.0092 mL	32.0184 mL	80.0461 mL
	5 mM	0.6404 mL	3.2018 mL	6.4037 mL	16.0092 mL
	10 mM	0.3202 mL	1.6009 mL	3.2018 mL	8.0046 mL
	15 mM	0.2135 mL	1.0673 mL	2.1346 mL	5.3364 mL
	20 mM	0.1601 mL	0.8005 mL	1.6009 mL	4.0023 mL
	25 mM	0.1281 mL	0.6404 mL	1.2807 mL	3.2018 mL
	30 mM	0.1067 mL	0.5336 mL	1.0673 mL	2.6682 mL
	40 mM	0.0800 mL	0.4002 mL	0.8005 mL	2.0012 mL
	50 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6009 mL
	60 mM	0.0534 mL	0.2668 mL	0.5336 mL	1.3341 mL
	80 mM	0.0400 mL	0.2001 mL	0.4002 mL	1.0006 mL

Tanshindiol C Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tanshindiol C97465-71-9Histone MethyltransferaseKeap1-Nrf2SirtuinNrf2Prdx1oxLDLABCA1atherosclerosisEZH2anti-cancer activityA667G mutant EZH2H3K27me3Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Tanshindiol C

Customer Review

Tanshindiol C Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Methyltransferase Isoform Specific Products:

View All Sirtuin Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Tanshindiol C Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Tanshindiol C Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report