TFEB activator 2

Cat. No.: HY-161724 Purity: 98.84%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

TFEB activator 2 (compound 37) exhibits TFEB activation and lysosome biogenesis capabilities. TFEB activator 2 has effect on TFEB nuclear translocation with 72% (10μM 3h) and 94 % (30 μM 3h). TFEB activator 2 plays an important role in Alzheimer’s disease (AD). TFEB activator 2 can pass blood brain barrier.

For research use only. We do not sell to patients.

TFEB activator 2 Chemical Structure

CAS No. : 3027414-04-3

Size		Stock
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution		Check price and availability
Solution
10 mM * 1 mL in DMSO		Check price and availability
Solid
5 mg		Check price and availability
10 mg		Check price and availability
25 mg		Check price and availability
50 mg		Check price and availability

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

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Description

Molecular Weight

436.52

Formula

C₂₆H₂₉FN₂O₃

CAS No.

3027414-04-3

Appearance

Solid

Color

White to off-white

SMILES

FC1=CC2=C(C=C1)NC=C2CCC3C4=C(CCN3CC5CCOCC5)C=C6OCOC6=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (229.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2908 mL	11.4542 mL	22.9085 mL
5 mM	0.4582 mL	2.2908 mL	4.5817 mL
10 mM	0.2291 mL	1.1454 mL	2.2908 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.45 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (11.45 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.84%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (256 KB) HNMR (137 KB) RP-HPLC (139 KB) LCMS (233 KB)

Handling Instructions (2659 KB)

References

[1]. Jianhui Zhou, et al. Discovery and Optimization of Tetrahydroisoquinoline Derivatives To Enhance Lysosome Biogenesis as Preclinical Candidates for the Treatment of Alzheimer’s Disease. J Med Chem. 2024 Jun 13;67(11):8836-8861. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2908 mL	11.4542 mL	22.9085 mL	57.2711 mL
	5 mM	0.4582 mL	2.2908 mL	4.5817 mL	11.4542 mL
	10 mM	0.2291 mL	1.1454 mL	2.2908 mL	5.7271 mL
	15 mM	0.1527 mL	0.7636 mL	1.5272 mL	3.8181 mL
	20 mM	0.1145 mL	0.5727 mL	1.1454 mL	2.8636 mL
	25 mM	0.0916 mL	0.4582 mL	0.9163 mL	2.2908 mL
	30 mM	0.0764 mL	0.3818 mL	0.7636 mL	1.9090 mL
	40 mM	0.0573 mL	0.2864 mL	0.5727 mL	1.4318 mL
	50 mM	0.0458 mL	0.2291 mL	0.4582 mL	1.1454 mL
	60 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9545 mL
	80 mM	0.0286 mL	0.1432 mL	0.2864 mL	0.7159 mL
	100 mM	0.0229 mL	0.1145 mL	0.2291 mL	0.5727 mL

TFEB activator 2 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TFEB activator 23027414-04-3TFEB activator2TFEB activator-2Amyloid-ββ-amyloid peptideAβAbetaAlzheimer’s diseaselysosomeHela cellsSH-SY5Y cellInhibitorinhibitorinhibit

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TFEB activator 2

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