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THAM  (Synonyms: Tris; Tris(hydroxymethyl)aminomethane)

Cat. No.: HY-D0227 Purity: 99.74%
COA Handling Instructions

Molarity Calculator

THAM (Tris) is a biologically inert amino alcohol of low toxicity, which buffers carbon dioxide and acids in vitro and in vivo. THAM is an effective amine compound for pH control in the physiological range.

For research use only. We do not sell to patients.

THAM Chemical Structure

THAM Chemical Structure

CAS No. : 77-86-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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 USD 28 In-stock
Solution
10 mM * 1 mL in DMSO USD 28 In-stock
Solid
25 g USD 25 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of THAM:

THAM Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

THAM (Tris) is a biologically inert amino alcohol of low toxicity, which buffers carbon dioxide and acids in vitro and in vivo. THAM is an effective amine compound for pH control in the physiological range[1].

In Vivo

THAM (9.2 mEq/kg/L, i.v.; 1 and 3 h) reduces arterial carbon dioxide tension (PaCO2) levels and increased pH above 7.4 during infusion in pigs, but after cessation of infusion, PaCO2 increases above control levels and pulmonary artery pressure and pulmonary vascular resistance decreases in pigs[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

121.14

Formula

C4H11NO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OCC(CO)(N)CO
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature, keep dry and cool
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (412.75 mM; Need ultrasonic)

DMSO : 25 mg/mL (206.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.2549 mL 41.2746 mL 82.5491 mL
5 mM 1.6510 mL 8.2549 mL 16.5098 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.74%

COA (249 KB) HNMR (150 KB) GC (179 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 8.2549 mL 41.2746 mL 82.5491 mL 206.3728 mL
5 mM 1.6510 mL 8.2549 mL 16.5098 mL 41.2746 mL
10 mM 0.8255 mL 4.1275 mL 8.2549 mL 20.6373 mL
15 mM 0.5503 mL 2.7516 mL 5.5033 mL 13.7582 mL
20 mM 0.4127 mL 2.0637 mL 4.1275 mL 10.3186 mL
25 mM 0.3302 mL 1.6510 mL 3.3020 mL 8.2549 mL
30 mM 0.2752 mL 1.3758 mL 2.7516 mL 6.8791 mL
40 mM 0.2064 mL 1.0319 mL 2.0637 mL 5.1593 mL
50 mM 0.1651 mL 0.8255 mL 1.6510 mL 4.1275 mL
60 mM 0.1376 mL 0.6879 mL 1.3758 mL 3.4395 mL
80 mM 0.1032 mL 0.5159 mL 1.0319 mL 2.5797 mL
100 mM 0.0825 mL 0.4127 mL 0.8255 mL 2.0637 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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THAM Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

THAM77-86-1Tris Tris(hydroxymethyl)aminomethaneBiochemical Assay ReagentsinertaminopHcontrolacidaemiaInhibitorinhibitorinhibit

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