Topoisomerase II inhibitor 13

Name: Topoisomerase II inhibitor 13
Brand: MedChemExpress
SKU: HY-153401
Rating: 4.5 (1 reviews)

Cat. No.: HY-153401 Purity: 99.26%: FAQs
Data Sheet SDS COA Handling Instructions

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Topoisomerase II inhibitor 13 is a topoisomerase II (Topo II) inhibitor. Topoisomerase II inhibitor 13 shows antiproliferative activity against several cancer cells. Topoisomerase II inhibitor 13 induces cancer cells apoptosis.

For research use only. We do not sell to patients.

Topoisomerase II inhibitor 13 Chemical Structure

CAS No. : 451515-89-2

Size	Price	Stock	Quantity
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Topoisomerase Topo I Topo II

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Topoisomerase II

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	25.33 μM Compound: 3n	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay Antiproliferative activity against human A549 cells after 48 hrs by MTT assay	[PMID: 26649766]
HEK293	IC₅₀	7.87 μM Compound: 3n	Cytotoxicity against HEK293 cells after 48 hrs by MTT assay Cytotoxicity against HEK293 cells after 48 hrs by MTT assay	[PMID: 26649766]
HeLa	IC₅₀	3.15 μM Compound: 3n	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 26649766]
HL-60	IC₅₀	> 0.73 μM Compound: 3n	Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay	[PMID: 26649766]
HL60/MX2	IC₅₀	1.82 μM Compound: 3n	Antiproliferative activity against human HL60/MX2 cells after 48 hrs by MTT assay Antiproliferative activity against human HL60/MX2 cells after 48 hrs by MTT assay	[PMID: 26649766]
K562	IC₅₀	1.8 μM Compound: 3n	Antiproliferative activity against human K562 cells after 48 hrs by MTT assay Antiproliferative activity against human K562 cells after 48 hrs by MTT assay	[PMID: 26649766]
Raji	IC₅₀	1.87 μM Compound: 3n	Antiproliferative activity against human Raji cells after 48 hrs by MTT assay Antiproliferative activity against human Raji cells after 48 hrs by MTT assay	[PMID: 26649766]

In Vitro

Topoisomerase II inhibitor 13 (compound 3m) (20 μM, 50 μM) exerts strong inhibitory effect at 20 μM, and almost completely inhibits Topo II activity^[1].
Topoisomerase II inhibitor 13 inhibits cancer cells proliferation with IC₅₀s of 25.85 μM (MDA-MB-231), 1.82 μM (A549), 10.38 μM (K562), 1.73 μM (Raji), 1.23 μM (HL-60), and 0.87 μM (HL-60/MX2), respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

413.48

Formula

C₂₂H₂₃N₉

CAS No.

451515-89-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

N#CC1=NC2=C(N(CCCN(CC)CC)C(N)=C2C3=NC4=C(N3)C=CC=C4)N=C1C#N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (30.23 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4185 mL	12.0925 mL	24.1850 mL
5 mM	0.4837 mL	2.4185 mL	4.8370 mL
10 mM	0.2418 mL	1.2092 mL	2.4185 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.26%

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Data Sheet (276 KB) SDS (251 KB)

COA (255 KB) HNMR (284 KB) LCMS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Li P H, et al. Synthesis and mechanism studies of 1, 3-benzoazolyl substituted pyrrolo [2, 3-b] pyrazine derivatives as nonintercalative topoisomerase II catalytic inhibitors. Journal of Medicinal Chemistry, 2016, 59(1): 238-252.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4185 mL	12.0925 mL	24.1850 mL	60.4624 mL
	5 mM	0.4837 mL	2.4185 mL	4.8370 mL	12.0925 mL
	10 mM	0.2418 mL	1.2092 mL	2.4185 mL	6.0462 mL
	15 mM	0.1612 mL	0.8062 mL	1.6123 mL	4.0308 mL
	20 mM	0.1209 mL	0.6046 mL	1.2092 mL	3.0231 mL
	25 mM	0.0967 mL	0.4837 mL	0.9674 mL	2.4185 mL
	30 mM	0.0806 mL	0.4031 mL	0.8062 mL	2.0154 mL