2. Neuronal Signaling
  3. Monoamine Oxidase
  4. Tranylcypromine hydrochloride

Tranylcypromine hydrochloride  (Synonyms: SKF 385 hydrochloride)

Cat. No.: HY-17447A Purity: 99.90%
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Tranylcypromine hydrochloride (SKF 385 hydrochloride) is an irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) and monoamine oxidase (MAO). Tranylcypromine hydrochloride inhibits LSD1, MAO A and MAO B with IC50s of 20.7, 2.3 and 0.95 μM, respectively. Tranylcypromine hydrochloride can be used for the research of depression.

For research use only. We do not sell to patients.

Tranylcypromine hydrochloride Chemical Structure

Tranylcypromine hydrochloride Chemical Structure

CAS No. : 1986-47-6

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Based on 4 publication(s) in Google Scholar

Other Forms of Tranylcypromine hydrochloride:

Tranylcypromine hydrochloride Related Antibodies

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MAO-A MAO-B

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  • Purity & Documentation

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Description

Tranylcypromine hydrochloride (SKF 385 hydrochloride) is an irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) and monoamine oxidase (MAO). Tranylcypromine hydrochloride inhibits LSD1, MAO A and MAO B with IC50s of 20.7, 2.3 and 0.95 μM, respectively. Tranylcypromine hydrochloride can be used for the research of depression[1][2][3].

IC50 & Target

MAO-A

2.3 μM (IC50)

MAO-B

0.95 μM (IC50)

In Vitro

Tranylcypromine hydrochlorid (50 μM-5 mM; 1 h or 12-14 h) inhibits histone and nucleosomal demethylation[1].
Tranylcypromine hydrochlorid (2 μM; 3 h) shows a specific derepression of OCT4 transcription[1].
Tranylcypromine hydrochlorid (0-100 μM; 15 min) shows IC50 values of 20.7, 2.3 and 0.95 μM for LSD1, MAO A and MAO B, respectively[2].
Tranylcypromine hydrochlorid (0-800 μM) shows Ki values of 242.7, 101.9 and 16 μM for LSD1, MAO A and MAO B, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tranylcypromine hydrochloride Related Antibodies

Western Blot Analysis[1]

Cell Line: Sf21 insect cell line
Concentration: 50 μM, 200 μM, 1 mM and 5 mM
Incubation Time: 12-14 hours or 1 hour
Result: Showed inhibitory activities of histone H3K4 demethylation and nucleosomal demethylation.

RT-PCR[1]

Cell Line: P19 EC cell line
Concentration: 2 μM
Incubation Time: 3 hours
Result: Decreased Oct4 mRNA levels.
In Vivo

Tranylcypromine hydrochlorid (3 mg/kg; i.p. once daily for 3 days) decreases LPS-mediated microglial activation and proinflammatory cytokine COX-2 and IL-6 levels in wild-type mice[3].
Tranylcypromine hydrochlorid (3 mg/kg; i.p. once daily for 7 days) down-regulates Aβ-mediate microglial activation in 5xFAD mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wild-type mice[3]
Dosage: 3 mg/kg
Administration: Intraperitoneal injection; 3 mg/kg once daily for 3 days
Result: Significantly down-regulated LPS-stimulated microglial activation in the cortex and Hippocampus, and LPS-induced astrocyte activation only in the cortex. Reduced LPS-induced COX-2 levels in hippocampus CA1, decreased LPS-evoked IL-6 levels in the cortex and hippocampus CA1 and suppressed LPS-mediated IL-1β levels in the cortex.
Animal Model: 5xFAD mice[3]
Dosage: 3 mg/kg
Administration: Intraperitoneal injection; 3 mg/kg once daily for 7 days
Result: Differentially regulated microglial and astrocyte activation in this mouse model of AD.
Molecular Weight

169.65

Formula

C9H12ClN
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

N[C@H]1[C@H](C2=CC=CC=C2)C1.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (589.45 mM; Need ultrasonic)

DMSO : 100 mg/mL (589.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.8945 mL 29.4724 mL 58.9449 mL
5 mM 1.1789 mL 5.8945 mL 11.7890 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (14.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (14.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.90%

SDS (479 KB)

COA (252 KB) LCMS (88 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 5.8945 mL 29.4724 mL 58.9449 mL 147.3622 mL
5 mM 1.1789 mL 5.8945 mL 11.7890 mL 29.4724 mL
10 mM 0.5894 mL 2.9472 mL 5.8945 mL 14.7362 mL
15 mM 0.3930 mL 1.9648 mL 3.9297 mL 9.8241 mL
20 mM 0.2947 mL 1.4736 mL 2.9472 mL 7.3681 mL
25 mM 0.2358 mL 1.1789 mL 2.3578 mL 5.8945 mL
30 mM 0.1965 mL 0.9824 mL 1.9648 mL 4.9121 mL
40 mM 0.1474 mL 0.7368 mL 1.4736 mL 3.6841 mL
50 mM 0.1179 mL 0.5894 mL 1.1789 mL 2.9472 mL
60 mM 0.0982 mL 0.4912 mL 0.9824 mL 2.4560 mL
80 mM 0.0737 mL 0.3684 mL 0.7368 mL 1.8420 mL
100 mM 0.0589 mL 0.2947 mL 0.5894 mL 1.4736 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Tranylcypromine hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tranylcypromine1986-47-6SKF 385SKF385SKF-385Monoamine OxidaseMAOsmall molecularmethylationdemethylationP19 ECSf21neuroinflammationmicrogliaamyloid betaLPSInhibitorinhibitorinhibit

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