2. Stem Cell/Wnt
  3. YAP
  4. TRULI

TRULI (Lats-IN-1) is a potent and ATP-competitive inhibitor of Lats1 and Lats2 kinases. TRULI promotes Yap-dependent proliferation in postmitotic mammalian tissues.

For research use only. We do not sell to patients.

TRULI Chemical Structure

TRULI Chemical Structure

CAS No. : 1424635-83-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 198 In-stock
Solution
10 mM * 1 mL in DMSO USD 198 In-stock
Solid
1 mg USD 85 In-stock
5 mg USD 150 In-stock
10 mg USD 230 In-stock
50 mg USD 600 In-stock
100 mg USD 850 In-stock
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500 mg   Get quote  

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Customer Review

Based on 16 publication(s) in Google Scholar

TRULI Related Antibodies

Top Publications Citing Use of Products

    TRULI purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2023 Mar 27;19(3):e1011272.  [Abstract]

    TRULI ( 30 μM; 24 h) has a significant inhibitory effect on the expression of CSPG4 in HeLa and HT-29 cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    TRULI (Lats-IN-1) is a potent and ATP-competitive inhibitor of Lats1 and Lats2 kinases. TRULI promotes Yap-dependent proliferation in postmitotic mammalian tissues[1].

    In Vitro

    The IC50 for TRULI increases with the ATP concentration[1].
    TRULI (10 μM; 24 hours) interferes with the ability of Lats kinases to phosphorylate Yap, with an EC50 of 510 nM[1].
    TRULI causes Yap-dependent proliferation of murine supporting cells in the inner ear, murine cardiomyocytes, and human Müller glia in retinal organoids[1].
    TRULI fosters both the G1-S and G2-M checkpoint transitions and yields supporting cells capable of transdifferentiation[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TRULI Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: MCF 10A cells
    Concentration: 10 μM
    Incubation Time: 24 hours
    Result: Decreased the phosphorylation of Yap at residue S127.
    Molecular Weight

    334.39

    Formula

    C18H14N4OS
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(C1=CNC2=NC=CC=C12)/N=C(SC=C3)/N3CC4=CC=CC=C4
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (149.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9905 mL 14.9526 mL 29.9052 mL
    5 mM 0.5981 mL 2.9905 mL 5.9810 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.98%

    COA (253 KB) RP-HPLC (209 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.9905 mL 14.9526 mL 29.9052 mL 74.7630 mL
    5 mM 0.5981 mL 2.9905 mL 5.9810 mL 14.9526 mL
    10 mM 0.2991 mL 1.4953 mL 2.9905 mL 7.4763 mL
    15 mM 0.1994 mL 0.9968 mL 1.9937 mL 4.9842 mL
    20 mM 0.1495 mL 0.7476 mL 1.4953 mL 3.7381 mL
    25 mM 0.1196 mL 0.5981 mL 1.1962 mL 2.9905 mL
    30 mM 0.0997 mL 0.4984 mL 0.9968 mL 2.4921 mL
    40 mM 0.0748 mL 0.3738 mL 0.7476 mL 1.8691 mL
    50 mM 0.0598 mL 0.2991 mL 0.5981 mL 1.4953 mL
    60 mM 0.0498 mL 0.2492 mL 0.4984 mL 1.2460 mL
    80 mM 0.0374 mL 0.1869 mL 0.3738 mL 0.9345 mL
    100 mM 0.0299 mL 0.1495 mL 0.2991 mL 0.7476 mL
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    TRULI Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TRULI1424635-83-5Lats-IN-1YAPYes-associated proteinInhibitorinhibitorinhibit

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