Tubulin polymerization-IN-56

Name: Tubulin polymerization-IN-56
Brand: MedChemExpress
SKU: HY-155362
Rating: 4.5 (1 reviews)

Cat. No.: HY-155362 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tubulin polymerization-IN-56 (compound 8l), an indazole derivative, is a potent tubulin polymerization inhibitor through interacting with the colchicine site, resulting in cell cycle arrest and cellular apoptosis. polymerization-IN-56 reduces cell migration and leads to more potent inhibition of tumor growth in vivo.

For research use only. We do not sell to patients.

Tubulin polymerization-IN-56 Chemical Structure

CAS No. : 2966790-98-5

Size	Price	Stock	Quantity
5 mg	USD 260	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 840	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1350	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2160	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

Molecular Weight

411.88

Formula

C₂₂H₂₂ClN₃O₃

CAS No.

2966790-98-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

CC1=CC=C(C2=CC=C3C(N(C4=CC(OC)=C(OC)C(OC)=C4)N=C3)=C2)C=N1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 40 mg/mL (97.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4279 mL	12.1395 mL	24.2789 mL
5 mM	0.4856 mL	2.4279 mL	4.8558 mL
10 mM	0.2428 mL	1.2139 mL	2.4279 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 4 mg/mL (9.71 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 4 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 4 mg/mL (9.71 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 4 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 4 mg/mL (9.71 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 4 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (254 KB) HNMR (155 KB) RP-HPLC (152 KB) LCMS (218 KB)

Handling Instructions (2659 KB)

References

[1]. Ying-Jie Cui, et al. The discovery of water-soluble indazole derivatives as potent microtubule polymerization inhibitors. Eur J Med Chem. 2023 Oct 18:262:115870. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4279 mL	12.1395 mL	24.2789 mL	60.6973 mL
	5 mM	0.4856 mL	2.4279 mL	4.8558 mL	12.1395 mL
	10 mM	0.2428 mL	1.2139 mL	2.4279 mL	6.0697 mL
	15 mM	0.1619 mL	0.8093 mL	1.6186 mL	4.0465 mL
	20 mM	0.1214 mL	0.6070 mL	1.2139 mL	3.0349 mL
	25 mM	0.0971 mL	0.4856 mL	0.9712 mL	2.4279 mL
	30 mM	0.0809 mL	0.4046 mL	0.8093 mL	2.0232 mL
	40 mM	0.0607 mL	0.3035 mL	0.6070 mL	1.5174 mL
	50 mM	0.0486 mL	0.2428 mL	0.4856 mL	1.2139 mL
	60 mM	0.0405 mL	0.2023 mL	0.4046 mL	1.0116 mL
	80 mM	0.0303 mL	0.1517 mL	0.3035 mL	0.7587 mL