Tyrosylleucine TFA (Synonyms: Tyr-Leu TFA)

Name: Tyrosylleucine TFA
Brand: MedChemExpress
SKU: HY-122794A
Rating: 4.5 (1 reviews)

Cat. No.: HY-122794A Purity: 98.05%: FAQs
Data Sheet SDS COA Handling Instructions

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Tyrosylleucine (Tyr-Leu, YL) TFA, an orally active dipeptide, exhibits a potent antidepressant-like activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Tyrosylleucine TFA Chemical Structure

CAS No. : 66852-01-5

Size	Price	Stock	Quantity
10 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Tyrosylleucine TFA:

Tyrosylleucine Get quote

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Description

Tyrosylleucine (Tyr-Leu, YL) TFA, an orally active dipeptide, exhibits a potent antidepressant-like activity^[1].

In Vitro

Tyrosylleucine (Tyr-Leu, YL) increases the amount of cells expressing c-Fos, a marker for neuronal activity, in the dentate gyrus of the hippocampus^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tyrosylleucine (Tyr-Leu, YL) dose-dependently exhibits potent anxiolytic-like activity (0.1-1 mg/kg, i.p.)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

408.37

Formula

C₁₇H₂₃F₃N₂O₆

CAS No.

66852-01-5

Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (244.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4488 mL	12.2438 mL	24.4876 mL
5 mM	0.4898 mL	2.4488 mL	4.8975 mL
10 mM	0.2449 mL	1.2244 mL	2.4488 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.05%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (250 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Takafumi Mizushige, et al. Dipeptide tyrosyl-leucine exhibits antidepressant-like activity in mice. Sci Rep. 2020 Feb 10;10(1):2257. [Content Brief]

[2]. Norimasa Kanegawa, et al. Dipeptide Tyr-Leu (YL) exhibits anxiolytic-like activity after oral administration via activating serotonin 5-HT1A, dopamine D1 and GABAA receptors in mice. FEBS Lett. 2010 Feb 5;584(3):599-604. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4488 mL	12.2438 mL	24.4876 mL	61.2190 mL
	5 mM	0.4898 mL	2.4488 mL	4.8975 mL	12.2438 mL
	10 mM	0.2449 mL	1.2244 mL	2.4488 mL	6.1219 mL
	15 mM	0.1633 mL	0.8163 mL	1.6325 mL	4.0813 mL
	20 mM	0.1224 mL	0.6122 mL	1.2244 mL	3.0609 mL
	25 mM	0.0980 mL	0.4898 mL	0.9795 mL	2.4488 mL
	30 mM	0.0816 mL	0.4081 mL	0.8163 mL	2.0406 mL
	40 mM	0.0612 mL	0.3061 mL	0.6122 mL	1.5305 mL
	50 mM	0.0490 mL	0.2449 mL	0.4898 mL	1.2244 mL
	60 mM	0.0408 mL	0.2041 mL	0.4081 mL	1.0203 mL
	80 mM	0.0306 mL	0.1530 mL	0.3061 mL	0.7652 mL
	100 mM	0.0245 mL	0.1224 mL	0.2449 mL	0.6122 mL

Tyrosylleucine TFA Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tyrosylleucine66852-01-5Tyr-LeuOtherstyrosineleucinec-FosantidepressantInhibitorinhibitorinhibit

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Tyrosylleucine TFA (Synonyms: Tyr-Leu TFA)

Customer Review

Other Forms of Tyrosylleucine TFA:

Tyrosylleucine TFA Related Antibodies

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•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

Tyrosylleucine TFA Related Antibodies

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Complete Stock Solution Preparation Table

Tyrosylleucine TFA Related Classifications

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