Verdinexor (Synonyms: KPT-335)

Name: Verdinexor
Brand: MedChemExpress
SKU: HY-15970
Rating: 5.0 (2 reviews)

Cat. No.: HY-15970 Purity: 99.91%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Verdinexor (KPT-335) is an orally active inhibitor for selective inhibitor of nuclear export (SINE). Verdinexor inhibits the respiratory syncytial virus A2 (RSV A2) replication with an IC₅₀ of 0.96 µM. Verdinexor inhibits the function of nuclear export protein Exportin 1 (XPO1/CRM1), inhibiting pro-inflammatory responses. Verdinexor exhibits antitumor effciency.

For research use only. We do not sell to patients.

Verdinexor Chemical Structure

CAS No. : 1392136-43-4

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Verdinexor

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
MDCK	CC₅₀	31.77 μM Compound: Verdinexor	Cytotoxicity against MDCK cells assessed as cell death after 72 hrs by MTT assay Cytotoxicity against MDCK cells assessed as cell death after 72 hrs by MTT assay	[PMID: 30753063]
SH-SY5Y	IC₅₀	0.3 μM Compound: 12; KPT-335	Antiproliferative activity against human SH-SY5Y cells by CCK-8 assay Antiproliferative activity against human SH-SY5Y cells by CCK-8 assay	[PMID: 34669417]
SK-N-BE(2)	IC₅₀	1.4 μM Compound: 12; KPT-335	Antiproliferative activity against human SK-N-BE(2) cells by CCK-8 assay Antiproliferative activity against human SK-N-BE(2) cells by CCK-8 assay	[PMID: 34669417]

In Vitro

Verdinexor (0-10 µM, 72 h) inhibits the proliferation of Jurkat cell and diffuse large B cell lymphoma (DLBCL) cells OCI-Ly3, OCI-Ly10 and CLBL1 with IC₅₀s of 0.3 nM, 2.1 nM, 41.8 nM, and 8.5 nM, respectively^[1].
Verdinexor (0-10 µM, 24 h) induces apoptosis in CLBL1 cell and primary canine DLBCL cell^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	OCI-Ly3, OCI-Ly10 and CLBL1
Concentration:	0-10 µM
Incubation Time:	72 h
Result:	Inhibited the proliferation.

Apoptosis Analysis^[1]

Cell Line:	CLBL1 and primary canine DLBCL cell
Concentration:	0-10 µM
Incubation Time:	24 h
Result:	Induced apoptosis.

In Vivo

Verdinexor (1-2 mg/kg, po, twice a week for 4 weeks) exhibits antitumor effect against non-Hodgkin lymphoma, but exhibits hepatotoxicity in dogs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Dog non-Hodgkin lymphoma models^[1]
Dosage:	1-2 mg/kg
Administration:	po, twice a week for 4 weeks
Result:	Stabilized or inhibited the tumor progression. Induced anorexia, weight loss, vomiting, and diarrhea.

Molecular Weight

442.32

Formula

C₁₈H₁₂F₆N₆O

CAS No.

1392136-43-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NNC1=NC=CC=C1)/C=C\N2N=C(C3=CC(C(F)(F)F)=CC(C(F)(F)F)=C3)N=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (226.08 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2608 mL	11.3040 mL	22.6081 mL
5 mM	0.4522 mL	2.2608 mL	4.5216 mL
10 mM	0.2261 mL	1.1304 mL	2.2608 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (251 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. London CA, et al., Preclinical evaluation of the novel, orally bioavailable Selective Inhibitor of Nuclear Export (SINE) KPT-335 in spontaneous canine cancer: results of a phase I study. PLoS One. 2014 Feb 4;9(2):e87585. [Content Brief]

[2]. Jorquera PA, et al., Verdinexor (KPT-335), a Selective Inhibitor of Nuclear Export, Reduces Respiratory Syncytial Virus Replication In Vitro. J Virol. 2019 Feb 5;93(4):e01684-18. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2608 mL	11.3040 mL	22.6081 mL	56.5202 mL
	5 mM	0.4522 mL	2.2608 mL	4.5216 mL	11.3040 mL
	10 mM	0.2261 mL	1.1304 mL	2.2608 mL	5.6520 mL
	15 mM	0.1507 mL	0.7536 mL	1.5072 mL	3.7680 mL
	20 mM	0.1130 mL	0.5652 mL	1.1304 mL	2.8260 mL
	25 mM	0.0904 mL	0.4522 mL	0.9043 mL	2.2608 mL
	30 mM	0.0754 mL	0.3768 mL	0.7536 mL	1.8840 mL
	40 mM	0.0565 mL	0.2826 mL	0.5652 mL	1.4130 mL
	50 mM	0.0452 mL	0.2261 mL	0.4522 mL	1.1304 mL
	60 mM	0.0377 mL	0.1884 mL	0.3768 mL	0.9420 mL
	80 mM	0.0283 mL	0.1413 mL	0.2826 mL	0.7065 mL
	100 mM	0.0226 mL	0.1130 mL	0.2261 mL	0.5652 mL

Verdinexor Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Verdinexor (Synonyms: KPT-335)

Customer Review

Verdinexor Related Antibodies

2 Publications Citing Use of MCE Verdinexor

Featured Recommendations

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•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Verdinexor Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Verdinexor Related Classifications

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