2. Cell Cycle/DNA Damage
  3. Deubiquitinase
  4. XL 188

XL 188 is a potent and selective USP7 inhibitor with IC50 values of 90 nM and 193 nM for USP7 full-length and catalytic domain enzyme, respectively. XL 188 can be used in research of cancer.

For research use only. We do not sell to patients.

XL 188 Chemical Structure

XL 188 Chemical Structure

CAS No. : 2305045-76-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 150 In-stock
Solution
10 mM * 1 mL in DMSO USD 150 In-stock
Solid
1 mg USD 40 In-stock
5 mg USD 94 In-stock
10 mg USD 160 In-stock
25 mg USD 340 In-stock
50 mg USD 544 In-stock
100 mg USD 870 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

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Customer Review

Based on 1 publication(s) in Google Scholar

XL 188 Related Antibodies

Top Publications Citing Use of Products

View All Deubiquitinase Isoform Specific Products:

View All Isoforms
USP1 USP2 USP7 USP8 USP30 UCHL1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

XL 188 is a potent and selective USP7 inhibitor with IC50 values of 90 nM and 193 nM for USP7 full-length and catalytic domain enzyme, respectively. XL 188 can be used in research of cancer[1].

In Vitro

XL 188 (1-20 μM; 16 h; MCF7 cells) promotes USP7 dependent loss of HDM2 and increase of p53 and p21[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

XL 188 Related Antibodies

Western Blot Analysis[1]

Cell Line: MCF7 cells
Concentration: 1, 5, 10, and 20μM
Incubation Time: 16 hours
Result: Had the loss of HDM2 accompanied by an increase in downstream tumor suppressors p21 and p53.
Molecular Weight

574.71

Formula

C32H42N6O4
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NC1=CC(N=CN2CC3(O)CCN(C(C[C@H](C4=CC=CC=C4)C)=O)CC3)=C(C=C1)C2=O)CCN5CCN(C)CC5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (174.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7400 mL 8.7000 mL 17.4001 mL
5 mM 0.3480 mL 1.7400 mL 3.4800 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (4.35 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (4.35 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7400 mL 8.7000 mL 17.4001 mL 43.5002 mL
5 mM 0.3480 mL 1.7400 mL 3.4800 mL 8.7000 mL
10 mM 0.1740 mL 0.8700 mL 1.7400 mL 4.3500 mL
15 mM 0.1160 mL 0.5800 mL 1.1600 mL 2.9000 mL
20 mM 0.0870 mL 0.4350 mL 0.8700 mL 2.1750 mL
25 mM 0.0696 mL 0.3480 mL 0.6960 mL 1.7400 mL
30 mM 0.0580 mL 0.2900 mL 0.5800 mL 1.4500 mL
40 mM 0.0435 mL 0.2175 mL 0.4350 mL 1.0875 mL
50 mM 0.0348 mL 0.1740 mL 0.3480 mL 0.8700 mL
60 mM 0.0290 mL 0.1450 mL 0.2900 mL 0.7250 mL
80 mM 0.0218 mL 0.1088 mL 0.2175 mL 0.5438 mL
100 mM 0.0174 mL 0.0870 mL 0.1740 mL 0.4350 mL
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XL 188 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

XL 1882305045-76-3XL188XL-188DeubiquitinaseDUBsfull-lengthcatalytic domain enzymecancerMCF7 cellsp53p21HDM2Inhibitorinhibitorinhibit

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