α-Methyl-DL-tryptophan (Synonyms: α-Methyltryptophan)

Name: α-Methyl-DL-tryptophan
Brand: MedChemExpress
SKU: HY-W142080
Rating: 4.5 (1 reviews)

Cat. No.: HY-W142080 Purity: 99.92%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

α-Methyl-DL-tryptophan (α-Methyltryptophan), a tryptophan derivative, is a selective SLC6A14 blocker. In estrogen receptor (ER)-positive breast cancer cells, α-Methyl-DL-tryptophan inhibits mTOR and activates autophagy and apoptosis. α-Methyl-DL-tryptophan also has the effect of reducing weight.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

α-Methyl-DL-tryptophan Chemical Structure

CAS No. : 153-91-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

α-Methyl-DL-tryptophan (α-MT) (2.5 mM; 24-72 h) increases the levels of asparagine synthetase and CHOP mRNA in MCF-7 cells. α-Methyl-DL-tryptophan does not increase the levels of these mRNAs in SLC6A14-negative MB-231 (a cancer cell line) and MCF-10A (a non-malignant cell line) cells^[1].
α-Methyl-DL-tryptophan (2.5 mM; 48 h) induces cell autophagy in MCF-7 cells^[1].
α-Methyl-DL-tryptophan (2.5 mM; 24-72 h) inhibits mTOR in MCF-7 cells^[1].
α-Methyl-DL-tryptophan (2.5 mM; 48 h) induces apoptotic cell death in MCF-7 cells^[1].
α-Methyl-DL-tryptophan (2.5 mM; 48 h) has no significant effect on cell migration and invasion in ZR-75-1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	MCF-7 cells^[1]
Concentration:	2.5 mM
Incubation Time:	24 h, 48 h, 72 h
Result:	Increased the levels of asparagine synthetase and CHOP mRNAs.

Cell Autophagy Assay^[1]

Cell Line:	MCF-7 cells^[1]
Concentration:	2.5 mM
Incubation Time:	48 h
Result:	Induced autophagy as shown by punctate localization of constitutively expressed LC3 and presence of autophagosomes.

Apoptosis Analysis^[1]

Cell Line:	MCF-7 cells^[1]
Concentration:	2.5 mM
Incubation Time:	48 h
Result:	Apoptotic cell death was demonstrated by annexin V labeling and cleavage of lamin A.

Western Blot Analysis^[1]

Cell Line:	MCF-7 cells^[1]
Concentration:	2.5 mM
Incubation Time:	24 h, 48 h, 72 h
Result:	Inhibited mTOR as evident from decreased phosphorylation of S6 and S6 kinase.

In Vivo

α-Methyl-DL-tryptophan (α-MT) (2 mg/mL; administered in drinking water; continuously administered; throughout the experimental period) inhibits tumor growth in BALB/c nude mice inoculated with ZR-75-1 cells^[1].
α-Methyl-DL-tryptophan (1 mg/mL; administered in drinking water; continuously administered; for 1 week) reduces body weight in wild-type mice on a high-fat diet^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice (female) inoculated with ZR-75-1 cells^[1]
Dosage:	2 mg/mL
Administration:	Administered in drinking water
Result:	Reduced the growth of ZR-75-1 cells. Tumor size was significantly smaller than that in the control group.

Animal Model:	Wild-type C57BL/6 male mice fed a high-fat diet^[2]
Dosage:	1 mg/mL
Administration:	Administered in drinking water, continuously for 1weeks
Result:	Led to a decrease in body weight.

Molecular Weight

218.26

Formula

C₁₂H₁₄N₂O₂

CAS No.

153-91-3

Appearance

Solid

Color

White to light yellow

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (229.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 10 mg/mL (45.82 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5818 mL	22.9089 mL	45.8178 mL
5 mM	0.9164 mL	4.5818 mL	9.1636 mL
10 mM	0.4582 mL	2.2909 mL	4.5818 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (11.45 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (4.58 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 5

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (4.58 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 6

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1 mg/mL (4.58 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (283 KB) SDS (393 KB)

COA (254 KB) HNMR (248 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Karunakaran S, et al. SLC6A14 (ATB0,+) protein, a highly concentrative and broad specific amino acid transporter, is a novel and effective drug target for treatment of estrogen receptor-positive breast cancer. J Biol Chem. 2011 Sep 9;286(36):31830-8. [Content Brief]

[2]. Sathish Sivaprakasam, et al. α-Methyl-l-tryptophan as a weight-loss agent in multiple models of obesity in mice. Biochem J. 2021 Apr 16;478(7):1347-1358. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	4.5818 mL	22.9089 mL	45.8178 mL	114.5444 mL
	5 mM	0.9164 mL	4.5818 mL	9.1636 mL	22.9089 mL
	10 mM	0.4582 mL	2.2909 mL	4.5818 mL	11.4544 mL
	15 mM	0.3055 mL	1.5273 mL	3.0545 mL	7.6363 mL
	20 mM	0.2291 mL	1.1454 mL	2.2909 mL	5.7272 mL
	25 mM	0.1833 mL	0.9164 mL	1.8327 mL	4.5818 mL
	30 mM	0.1527 mL	0.7636 mL	1.5273 mL	3.8181 mL
	40 mM	0.1145 mL	0.5727 mL	1.1454 mL	2.8636 mL
DMSO	50 mM	0.0916 mL	0.4582 mL	0.9164 mL	2.2909 mL
	60 mM	0.0764 mL	0.3818 mL	0.7636 mL	1.9091 mL
	80 mM	0.0573 mL	0.2864 mL	0.5727 mL	1.4318 mL
	100 mM	0.0458 mL	0.2291 mL	0.4582 mL	1.1454 mL

α-Methyl-DL-tryptophan Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

α-Methyl-DL-tryptophan153-91-3α-MethyltryptophanmTORAutophagyApoptosisAmino Acid DerivativesMammalian target of RapamycinInhibitorinhibitorinhibit

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α-Methyl-DL-tryptophan (Synonyms: α-Methyltryptophan)

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α-Methyl-DL-tryptophan Related Antibodies

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References

Customer Review

α-Methyl-DL-tryptophan Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

α-Methyl-DL-tryptophan Related Classifications

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