2. Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related
  3. DNA/RNA Synthesis ADC Cytotoxin
  4. γ-Amanitin

γ-Amanitin an ADC cytotoxin and isolated from the mushroom. γ-Amanitin inhibits RNA polymerase II and disrupts synthesis of mRNA. γ-Amanitin shows similar effects to α-Amanitin and β-Amanitin. γ-Amanitin competitively binds to monoclonal antibody (mAb), with an IC50 of 163.1 ng/mL. γ-Amanitin is toxic to a variety of cells.

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γ-Amanitin Chemical Structure

γ-Amanitin Chemical Structure

CAS No. : 21150-23-2

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Based on 1 publication(s) in Google Scholar

Other Forms of γ-Amanitin:

γ-Amanitin Related Antibodies

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  • Customer Review

Description

γ-Amanitin an ADC cytotoxin and isolated from the mushroom. γ-Amanitin inhibits RNA polymerase II and disrupts synthesis of mRNA. γ-Amanitin shows similar effects to α-Amanitin and β-Amanitin. γ-Amanitin competitively binds to monoclonal antibody (mAb), with an IC50 of 163.1 ng/mL. γ-Amanitin is toxic to a variety of cells[1][2][3].

IC50 & Target[1]

Traditional Cytotoxic Agents

 

In Vitro

γ-Amanitin (0.3-100 μM, 24 h) concentration-dependently reduces viability of eight human-derived cell lines[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

γ-Amanitin Related Antibodies

Cell Viability Assay[2]

Cell Line: HepG2, HCT-8, BGC-823, A549, SH-SY5Y, HEK-293, HUVEC and AC16
Concentration: from 0.3 to 100 μM
Incubation Time: 24 h
Result: Reduced cell viability.
Most sensitive to liver-derived HepG2, stomach-derived BGC-823, and kidney-derived HEK-293 cells (IC50: 9.12, 8.27, and 12.68 μM).
Least potent against lung-derived A549, heart-derived AC16, and intestinal-derived HCT-8 cells (IC50: >100 μM).
In Vivo

γ-amanitin (i.p.) is classified as a neutral amanitin with an LD50 in mice ranging from 0.2 to 0.5 mg/kg[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

902.97

Formula

C39H54N10O13S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=[S](C[C@H](NC(CNC1=O)=O)C(N[C@H](C2=O)CC(N)=O)=O)C(N3)=C(C[C@@H](C(NCC(N[C@H]1[C@@H](C)CC)=O)=O)NC([C@@H](NC([C@H](C4)N2C[C@@H]4O)=O)[C@@H](C)[C@@H](O)C)=O)C(C3=C5)=CC=C5O
Structure Classification
Initial Source

Amanita phalloides var. alba mushroom
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 1 mg/mL (1.11 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1075 mL 5.5373 mL 11.0746 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥90.0%

SDS (252 KB)

COA (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1075 mL 5.5373 mL 11.0746 mL 27.6864 mL
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γ-Amanitin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

γ-Amanitin21150-23-2DNA/RNA SynthesisADC CytotoxinADC PayloadmushroomADC cytotoxinRNA polymerase IImRNAprotein synthesisInhibitorinhibitorinhibit

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