14-Deoxyandrographolide

Name: 14-Deoxyandrographolide
Brand: MedChemExpress
SKU: HY-N4323
Rating: 4.5 (1 reviews)

Cat. No.: HY-N4323 Purity: 99.47%: FAQs
Data Sheet SDS COA Handling Instructions

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14-Deoxyandrographolide is a diterpene with calcium channel blocking activity and acts as a uterine smooth muscle relaxant. 14-Deoxyandrographolide stimulates the release of nitric oxide (NO) in endothelial cells. 14-Deoxyandrographolide gradually desensitizes liver cells to TNF-α mediated apoptosis by inducing the release of TNFRSF1A.

For research use only. We do not sell to patients.

14-Deoxyandrographolide Chemical Structure

CAS No. : 4176-97-0

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 14-Deoxyandrographolide:

14-Deoxyandrographolide (Standard) Get quote

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View All Calcium Channel Isoform Specific Products:

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Caspase 3

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	GI₅₀	25.46 μM Compound: 9	Antiproliferative activity against human HL60 cells by trypan blue assay Antiproliferative activity against human HL60 cells by trypan blue assay	[PMID: 18357994]
NIH3T3	IC₅₀	176.3 μM Compound: 5	Antifibrotic activity against mouse NIH/3T3 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay Antifibrotic activity against mouse NIH/3T3 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay	[PMID: 30144698]
NTUB1	IC₅₀	> 100 μM Compound: 4	Cytotoxicity against human NTUB1 cells after 48 hrs by MTT assay Cytotoxicity against human NTUB1 cells after 48 hrs by MTT assay	[PMID: 25913115]
RAW264.7	IC₅₀	21.01 μM Compound: 1	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 22264489]

In Vitro

14-Deoxyandrographolide can reduce uterine smooth muscle contraction responses induced by 0.3 mM and 3.0 mM CaCl², with IC₅₀ values of 1.24 M and 5.94 M^[1].
14-Deoxyandrographolide (0-15 nM, 1 h) shows no cytotoxicity in primary liver cells and prevents TNF-α induced cell death^[2].
14-Deoxyandrographolide (5-15 nM, 1 h) inhibits the expression of caspase-3 in primary liver cells^[2].
14-Deoxyandrographolide (10 nM, 1 h) suppressed the aggregation of TRADD, FADD and caspase-8 in TNF-a sensitized liver cells and inhibited the formation of the DISC^[2].
14-Deoxyandrographolide (10 nM, 1 h) induces the release of TNFRSF1A from liver cells^[2].
14-Deoxyandrographolide (0-100 μM, 24-48 h) stimulates endothelial cells to produce NO in a concentration-dependent manner^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	Primary hepatocytes
Concentration:	0, 2.5, 5, 7.5, 10, 12.5, 15 nM
Incubation Time:	1 h
Result:	Reduced cell death rate.

Western Blot Analysis^[2]

Cell Line:	Primary hepatocytes
Concentration:	5, 10, 15 nM
Incubation Time:	1 h
Result:	Inhibited the expression of caspase-3 in a concentration-dependent manner.

In Vivo

14-Deoxyandrographolide (40 mg/kg, intraperitoneal injection, single dose) makes rat liver cells resistant to apoptosis induced by TNF-α by downregulating the formation of death-inducing signaling complex. It enhances the activity of microsomal Ca-ATPase and increases calcium ion influx into the microsomal lumen through the NO/cGMP pathway, which in turn promotes the release of TNFRSF1A^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats^[2]
Dosage:	40 mg/kg; single dose
Administration:	Intraperitoneal injection (i.p.)
Result:	Reduced ALT elevation caused by TNF-α/ActD, reduced the number of hepatocyte apoptosis, and reduced the amount of TNF-α retained in the liver.

Molecular Weight

334.45

Formula

C₂₀H₃₀O₄

CAS No.

4176-97-0

Appearance

Solid

Color

White to off-white

SMILES

O=C1OCC=C1CC[C@@H]2C(CC[C@]3([H])[C@](C)(CO)[C@H](O)CC[C@@]23C)=C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (299.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9900 mL	14.9499 mL	29.8998 mL
5 mM	0.5980 mL	2.9900 mL	5.9800 mL
10 mM	0.2990 mL	1.4950 mL	2.9900 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.47%

Select Batch:

Data Sheet (296 KB) SDS (251 KB)

COA (253 KB) HNMR (107 KB) CNMR (103 KB) RP-HPLC (249 KB) MS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Burgos RA, et al. Effect of 14-deoxyandrographolide on calcium-mediated rat uterine smooth muscle contractility. Phytother Res. 2003;17(9):1011-1015. [Content Brief]

[2]. Roy DN, et al. 14-Deoxyandrographolide desensitizes hepatocytes to tumour necrosis factor-alpha-induced apoptosis through calcium-dependent tumour necrosis factor receptor superfamily member 1A release via the NO/cGMP pathway. Br J Pharmacol. 2010 Aug;160(7):1823-43. [Content Brief]

[3]. C Y Zhang, et al. Effects of 14-deoxyandrographolide and 14-deoxy-11,12-didehydroandrographolide on nitric oxide production in cultured human endothelial cells. Phytother Res. 1999 Mar;13(2):157-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9900 mL	14.9499 mL	29.8998 mL	74.7496 mL
	5 mM	0.5980 mL	2.9900 mL	5.9800 mL	14.9499 mL
	10 mM	0.2990 mL	1.4950 mL	2.9900 mL	7.4750 mL
	15 mM	0.1993 mL	0.9967 mL	1.9933 mL	4.9833 mL
	20 mM	0.1495 mL	0.7475 mL	1.4950 mL	3.7375 mL
	25 mM	0.1196 mL	0.5980 mL	1.1960 mL	2.9900 mL
	30 mM	0.0997 mL	0.4983 mL	0.9967 mL	2.4917 mL
	40 mM	0.0747 mL	0.3737 mL	0.7475 mL	1.8687 mL
	50 mM	0.0598 mL	0.2990 mL	0.5980 mL	1.4950 mL
	60 mM	0.0498 mL	0.2492 mL	0.4983 mL	1.2458 mL
	80 mM	0.0374 mL	0.1869 mL	0.3737 mL	0.9344 mL
	100 mM	0.0299 mL	0.1495 mL	0.2990 mL	0.7475 mL