2. Cell Cycle/DNA Damage Apoptosis
  3. DNA/RNA Synthesis Apoptosis
  4. 2,4-D

2,4-D  (Synonyms: 2,4-Dichlorophenoxyacetic acid)

Cat. No.: HY-18572 Purity: 99.88%
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2,4-D (2, 4-dichlorophenoxyacetic acid) is a selective herbicide that can be orally active for the control of broad-leaved weeds. 2,4-D can induce apoptosis. 2,4-D inhibits DNA and protein synthesis, thereby preventing normal plant growth and development.

For research use only. We do not sell to patients.

2,4-D Chemical Structure

2,4-D Chemical Structure

CAS No. : 94-75-7

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Based on 1 publication(s) in Google Scholar

Other Forms of 2,4-D:

2,4-D Related Antibodies

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Description

2,4-D (2, 4-dichlorophenoxyacetic acid) is a selective herbicide that can be orally active for the control of broad-leaved weeds. 2,4-D can induce apoptosis. 2,4-D inhibits DNA and protein synthesis, thereby preventing normal plant growth and development[1][2][3].

In Vitro

2, 4-D (25-200 μM, 72 h) inhibits the cell viability of A549 and WI38 with IC50 values of 126 ± 2.25 µM and 115 ± 4.39 µM, respectively. Induces apoptosis by influencing the cell cycle[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2,4-D Related Antibodies

Cell Viability Assay[2]

Cell Line: A549, W138
Concentration: 25, 50, 75, 100, 150, 200 μM
Incubation Time: 72 h
Result: Inhibited cell viability in a dose-dependent manner.

Cell Cycle Analysis[2]

Cell Line: A549
Concentration: 50, 100, 150 μM
Incubation Time: 72 h
Result: The G0/G1 population decreased to about 57%, 43% and 31%, respectively.

Apoptosis Analysis[2]

Cell Line: A549, W138
Concentration: 100, 150 μM
Incubation Time: 72 h
Result: The early apoptotic population increased to 44% in A549 cells and 57% in WI38 cells, and the late apoptotic population increased to 18% in A549 cells and 10% in WI38 cells.

Western Blot Analysis[2]

Cell Line: A549, W138
Concentration: 100, 150 μM
Incubation Time: 72 h
Result: Up-regulated p53 and Bax proteins, down-regulated Bcl-2 and procaspase 3.
In Vivo

2,4-D (100 or 200 mg/kg, gavage for 30 days) has toxic effects on the reproductive system of male rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult Wistar male rats[3]
Dosage: 100 or 200 mg/kg
Administration: i.g.
Result: Decreased the testis, seminal vesicles and prostate relative weight.
Decreased the number of spermatozoa and sperm motility.
Increased the level of FSH and LH.
Molecular Weight

221.04

Formula

C8H6Cl2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)COC1=CC=C(Cl)C=C1Cl
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (452.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5241 mL 22.6203 mL 45.2407 mL
5 mM 0.9048 mL 4.5241 mL 9.0481 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.31 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (11.31 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.88%

SDS (761 KB)

COA (246 KB) HNMR (117 KB) RP-HPLC (209 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5241 mL 22.6203 mL 45.2407 mL 113.1017 mL
5 mM 0.9048 mL 4.5241 mL 9.0481 mL 22.6203 mL
10 mM 0.4524 mL 2.2620 mL 4.5241 mL 11.3102 mL
15 mM 0.3016 mL 1.5080 mL 3.0160 mL 7.5401 mL
20 mM 0.2262 mL 1.1310 mL 2.2620 mL 5.6551 mL
25 mM 0.1810 mL 0.9048 mL 1.8096 mL 4.5241 mL
30 mM 0.1508 mL 0.7540 mL 1.5080 mL 3.7701 mL
40 mM 0.1131 mL 0.5655 mL 1.1310 mL 2.8275 mL
50 mM 0.0905 mL 0.4524 mL 0.9048 mL 2.2620 mL
60 mM 0.0754 mL 0.3770 mL 0.7540 mL 1.8850 mL
80 mM 0.0566 mL 0.2828 mL 0.5655 mL 1.4138 mL
100 mM 0.0452 mL 0.2262 mL 0.4524 mL 1.1310 mL
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2,4-D Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2,4-D94-75-72,4-Dichlorophenoxyacetic acidDNA/RNA SynthesisApoptosisA549W138HerbicideFSHLHInhibitorinhibitorinhibit

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