2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. 2-Oxobutanoic acid

2-Oxobutanoic acid  (Synonyms: 2-Ketobutyrate)

Cat. No.: HY-W007926 Purity: 99.55%
COA Handling Instructions

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2-Oxobutanoic acid is a product in the enzymatic cleavage of cystathionine.

For research use only. We do not sell to patients.

2-Oxobutanoic acid Chemical Structure

2-Oxobutanoic acid Chemical Structure

CAS No. : 600-18-0

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 2-Oxobutanoic acid:

2-Oxobutanoic acid Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 2-Oxobutanoic acid

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

2-Oxobutanoic acid is a product in the enzymatic cleavage of cystathionine.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

2-Oxobutanoic acid (alpha-Ketobutyric acid) is a product in the enzymatic cleavage of cystathionine. 2-Oxobutanoic acid is a substance that is involved in the metabolism of many amino acids as well as propanoate metabolism and C-5 branched dibasic acid metabolism. 2-Oxobutanoic acid is also one of the degradation products of threonine. It can be converted into propionyl-CoA (and subsequently methylmalonyl CoA, which can be converted into succinyl CoA, a citric acid cycle intermediate), and thus enter the citric acid cycle[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-Oxobutanoic acid Related Antibodies
Molecular Weight

102.09

Formula

C4H6O3
CAS No.
Appearance

<30°C Solid,>34°C Liquid

Color

Colorless to off-white

SMILES

CCC(C(O)=O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (489.76 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 9.7953 mL 48.9764 mL 97.9528 mL
5 mM 1.9591 mL 9.7953 mL 19.5906 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (24.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (24.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.55%

COA (246 KB) HNMR (232 KB) LCMS (85 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 9.7953 mL 48.9764 mL 97.9528 mL 244.8820 mL
5 mM 1.9591 mL 9.7953 mL 19.5906 mL 48.9764 mL
10 mM 0.9795 mL 4.8976 mL 9.7953 mL 24.4882 mL
15 mM 0.6530 mL 3.2651 mL 6.5302 mL 16.3255 mL
20 mM 0.4898 mL 2.4488 mL 4.8976 mL 12.2441 mL
25 mM 0.3918 mL 1.9591 mL 3.9181 mL 9.7953 mL
30 mM 0.3265 mL 1.6325 mL 3.2651 mL 8.1627 mL
40 mM 0.2449 mL 1.2244 mL 2.4488 mL 6.1220 mL
50 mM 0.1959 mL 0.9795 mL 1.9591 mL 4.8976 mL
60 mM 0.1633 mL 0.8163 mL 1.6325 mL 4.0814 mL
80 mM 0.1224 mL 0.6122 mL 1.2244 mL 3.0610 mL
100 mM 0.0980 mL 0.4898 mL 0.9795 mL 2.4488 mL
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2-Oxobutanoic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Oxobutanoic acid600-18-02-KetobutyrateEndogenous MetaboliteInhibitorinhibitorinhibit

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