2. GPCR/G Protein
  3. P2Y Receptor
  4. 2-Methylthio-AMP

2-Methylthio-AMP  (Synonyms: 2-MeSAMP; 2-Methylthioadenosine 5′-monophosphate; 2-Methylthioadenosine 5′-phosphate)

Cat. No.: HY-125989 Purity: 98.94%
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2-Methylthio-AMP (2-MeSAMP) is a selective and direct P2Y12 antagonist. 2-Methylthio-AMP is an inhibitor of ADP-dependent platelet aggregation.

For research use only. We do not sell to patients.

2-Methylthio-AMP Chemical Structure

2-Methylthio-AMP Chemical Structure

CAS No. : 22140-20-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 352 In-stock
Solution
10 mM * 1 mL in DMSO USD 352 In-stock
Solid
5 mg USD 320 In-stock
10 mg USD 490 In-stock
25 mg USD 980 In-stock
50 mg USD 1550 In-stock
100 mg USD 2500 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of 2-Methylthio-AMP:

2-Methylthio-AMP Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 2-Methylthio-AMP

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

2-Methylthio-AMP (2-MeSAMP) is a selective and direct P2Y12 antagonist. 2-Methylthio-AMP is an inhibitor of ADP-dependent platelet aggregation[1][2][3].

IC50 & Target

P2Y12 Receptor

 

In Vitro

2-Methylthio-AMP (2-MeSAMP) inhibits agonist-mediated αIIbβ3 activation in platelets[1].
2-methylthio-AMP (50 μM; for 5 minutes) does not significantly inhibit thrombin, PAR1-AP, PAR4-AP, or ADP-mediated platelet Ca2+ mobilization. 2-methylthio-AMP fails to inhibit Ca2+ mobilization in P2Y12-deficient mouse platelets and does not raise cAMP or induce vasodilator-stimulated phosphoprotein phosphorylation in wild-type platelets[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-Methylthio-AMP Related Antibodies
Molecular Weight

393.31

Formula

C11H16N5O7PS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=P(O)(OC[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C(N=C(SC)N=C32)N)O1)O)O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (254.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5425 mL 12.7126 mL 25.4252 mL
5 mM 0.5085 mL 2.5425 mL 5.0850 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.36 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.36 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5425 mL 12.7126 mL 25.4252 mL 63.5631 mL
5 mM 0.5085 mL 2.5425 mL 5.0850 mL 12.7126 mL
10 mM 0.2543 mL 1.2713 mL 2.5425 mL 6.3563 mL
15 mM 0.1695 mL 0.8475 mL 1.6950 mL 4.2375 mL
20 mM 0.1271 mL 0.6356 mL 1.2713 mL 3.1782 mL
25 mM 0.1017 mL 0.5085 mL 1.0170 mL 2.5425 mL
30 mM 0.0848 mL 0.4238 mL 0.8475 mL 2.1188 mL
40 mM 0.0636 mL 0.3178 mL 0.6356 mL 1.5891 mL
50 mM 0.0509 mL 0.2543 mL 0.5085 mL 1.2713 mL
60 mM 0.0424 mL 0.2119 mL 0.4238 mL 1.0594 mL
80 mM 0.0318 mL 0.1589 mL 0.3178 mL 0.7945 mL
100 mM 0.0254 mL 0.1271 mL 0.2543 mL 0.6356 mL
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2-Methylthio-AMP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Methylthio-AMP22140-20-12-MeSAMP 2-Methylthioadenosine 5′-monophosphate 2-Methylthioadenosine 5′-phosphateP2Y ReceptorαIIbβ3plateletsthrombinPAR1-APPAR4-APADPCa2+P2Y12Inhibitorinhibitorinhibit

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