2'-O,4'-C-Methyleneguanosine  (Synonyms: LNA-G)

2′-O,4′-C-Methyleneguanosine (LNA-G) is a reverse guanine analogue, where LNA (locked nucleic acid) is a nucleic acid analogue. LNA modification can be used in a variety of applications such as effective binding affinity to complementary sequences and greater nuclease resistance than natural nucleotides, offering great potential for applications in disease diagnosis and research. LNA-G is also available via KOD DNA polymerase, which allows the integration of LNA-G nucleotides into the DNA strand^[1][2].

295.25

C₁₁H₁₃N₅O₅

207131-16-6

Solid

White to off-white

OC[C@]12[C@@H](O)[C@@H](OC2)[C@H](N3C(N=C(N)NC4=O)=C4N=C3)O1

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Rakesh N. Veedu, et al. Polymerase directed incorporation studies of LNA-G nucleoside 5′-triphosphate and primer extension involving all four LNAnucleotides. New J. Chem., 2010,34, 877-879

  [2]. Haase L, et al. Locked nucleic acid building blocks as versatile tools for advanced G-quadruplex design. Nucleic Acids Res. 2020 Oct 9;48(18):10555-10566.  [Content Brief]