2. Cell Cycle/DNA Damage
  3. Nucleoside Antimetabolite/Analog DNA/RNA Synthesis
  4. 2'-O,4'-C-Methyleneguanosine

2'-O,4'-C-Methyleneguanosine  (Synonyms: LNA-G)

Cat. No.: HY-W406070 Purity: 99.22%
SDS
COA
Handling Instructions Technical Support

2′-O,4′-C-Methyleneguanosine (LNA-G) is a reverse guanine analogue, where LNA (locked nucleic acid) is a nucleic acid analogue. LNA modification can be used in a variety of applications such as effective binding affinity to complementary sequences and greater nuclease resistance than natural nucleotides, offering great potential for applications in disease diagnosis and research. LNA-G is also available via KOD DNA polymerase, which allows the integration of LNA-G nucleotides into the DNA strand.

For research use only. We do not sell to patients.

2'-O,4'-C-Methyleneguanosine Chemical Structure

2'-O,4'-C-Methyleneguanosine Chemical Structure

CAS No. : 207131-16-6

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Based on 1 publication(s) in Google Scholar

2'-O,4'-C-Methyleneguanosine Related Antibodies

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  • Purity & Documentation

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Description

2′-O,4′-C-Methyleneguanosine (LNA-G) is a reverse guanine analogue, where LNA (locked nucleic acid) is a nucleic acid analogue. LNA modification can be used in a variety of applications such as effective binding affinity to complementary sequences and greater nuclease resistance than natural nucleotides, offering great potential for applications in disease diagnosis and research. LNA-G is also available via KOD DNA polymerase, which allows the integration of LNA-G nucleotides into the DNA strand[1][2].

Cellular Effect
Cell Line Type Value Description References
Huh-7 EC50
> 50 μM
Compound: 7
Antiviral activity against wild type Hepatitis C virus genotype 1b infected in human HuH7 cells after 72 hrs by luciferase reporter gene assay
Antiviral activity against wild type Hepatitis C virus genotype 1b infected in human HuH7 cells after 72 hrs by luciferase reporter gene assay
[PMID: 24815510]
Molecular Weight

295.25

Formula

C11H13N5O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@]12[C@@H](O)[C@@H](OC2)[C@H](N3C(N=C(N)NC4=O)=C4N=C3)O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 35 mg/mL (118.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3870 mL 16.9348 mL 33.8696 mL
5 mM 0.6774 mL 3.3870 mL 6.7739 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.22%

SDS (393 KB)

COA (252 KB) HNMR (272 KB) RP-HPLC (258 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3870 mL 16.9348 mL 33.8696 mL 84.6740 mL
5 mM 0.6774 mL 3.3870 mL 6.7739 mL 16.9348 mL
10 mM 0.3387 mL 1.6935 mL 3.3870 mL 8.4674 mL
15 mM 0.2258 mL 1.1290 mL 2.2580 mL 5.6449 mL
20 mM 0.1693 mL 0.8467 mL 1.6935 mL 4.2337 mL
25 mM 0.1355 mL 0.6774 mL 1.3548 mL 3.3870 mL
30 mM 0.1129 mL 0.5645 mL 1.1290 mL 2.8225 mL
40 mM 0.0847 mL 0.4234 mL 0.8467 mL 2.1169 mL
50 mM 0.0677 mL 0.3387 mL 0.6774 mL 1.6935 mL
60 mM 0.0564 mL 0.2822 mL 0.5645 mL 1.4112 mL
80 mM 0.0423 mL 0.2117 mL 0.4234 mL 1.0584 mL
100 mM 0.0339 mL 0.1693 mL 0.3387 mL 0.8467 mL
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2'-O,4'-C-Methyleneguanosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2'-O,4'-C-Methyleneguanosine207131-16-6LNA-GNucleoside Antimetabolite/AnalogDNA/RNA Synthesisguanine analogueLNALNA modificationKOD DNA polymerasedisease diagnosisInhibitorinhibitorinhibit

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