24-Methylenecycloartanyl ferulate

Cat. No.: HY-N8122 Purity: 98.05%: FAQs
Data Sheet SDS COA Handling Instructions

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24-Methylenecycloartanyl ferulate is a γ-oryzanol compound. 24-Methylenecycloartanyl ferulate promotes parvin-beta expression in human breast cancer cells. 24-Methylenecycloartanyl ferulate is a potential ATP-competitive Akt1 inhibitor (EC₅₀= 33.3μM).

For research use only. We do not sell to patients.

24-Methylenecycloartanyl ferulate Chemical Structure

CAS No. : 469-36-3

Size	Price	Stock	Quantity
5 mg	USD 230	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Akt1

33.3 μM (EC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	27 μM Compound: 5	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 15679326]
Raji	IC₅₀	15.8 nM Compound: 18	Inhibition of TPA-induced Epstein-Barr virus early antigen activation in Raji cells after 48 hrs Inhibition of TPA-induced Epstein-Barr virus early antigen activation in Raji cells after 48 hrs	[PMID: 17503850]

Molecular Weight

616.91

Formula

C₄₁H₆₀O₄

CAS No.

469-36-3

Appearance

Solid

Color

White to off-white

SMILES

CC1(C)[C@@H](OC(/C=C/C2=CC=C(O)C(OC)=C2)=O)CC[C@]3(C4)C54CC[C@]6(C)[C@@H]([C@H](C)CCC(C(C)C)=C)CC[C@](C)6[C@]5([H])CC[C@@]13[H]

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 12.5 mg/mL (20.26 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6210 mL	8.1049 mL	16.2098 mL
5 mM	0.3242 mL	1.6210 mL	3.2420 mL
10 mM	0.1621 mL	0.8105 mL	1.6210 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.03 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.05%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (248 KB) HNMR (117 KB) CNMR (147 KB) RP-HPLC (401 KB) MS (155 KB)

Handling Instructions (2659 KB)

References

[1]. Heon Woong Kim, et al. 24-Methylenecycloartanyl ferulate, a major compound of γ-oryzanol, promotes parvin-beta expression through an interaction with peroxisome proliferator-activated receptor-gamma 2 in human breast cancer cells. Biochem Biophys Res Commun. 2015 Dec 25;468(4):574-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6210 mL	8.1049 mL	16.2098 mL	40.5246 mL
	5 mM	0.3242 mL	1.6210 mL	3.2420 mL	8.1049 mL
	10 mM	0.1621 mL	0.8105 mL	1.6210 mL	4.0525 mL
	15 mM	0.1081 mL	0.5403 mL	1.0807 mL	2.7016 mL
	20 mM	0.0810 mL	0.4052 mL	0.8105 mL	2.0262 mL