3,4-Dicaffeoylquinic acid (Synonyms: 3,4-Di-O-caffeoylquinic acid; Isochlorogenic acid B)

Name: 3,4-Dicaffeoylquinic acid
Brand: MedChemExpress
SKU: HY-N0057
Rating: 5.0 (4 reviews)

Cat. No.: HY-N0057 Purity: 98.64%: FAQs
Data Sheet SDS COA Handling Instructions

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3,4-Dicaffeoylquinic acid (3,4-Di-O-caffeoylquinic acid), naturally isolated from Laggera alata, has antioxidative, DNA protective, neuroprotective and hepatoprotective properties. 3,4-Dicaffeoylquinic acid exerts apoptosis-mediated cytotoxicity and α-glucosidase inhibitory effects. 3,4-Dicaffeoylquinic acid possesses a unique mechanism of anti-influenza viral activity, that is, enhancing viral clearance by increasing TRAIL.

For research use only. We do not sell to patients.

3,4-Dicaffeoylquinic acid Chemical Structure

CAS No. : 14534-61-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 134	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 134	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 56	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 118	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 186	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 4 publication(s) in Google Scholar

Other Forms of 3,4-Dicaffeoylquinic acid:

3,4-Dicaffeoylquinic acid (Standard) Get quote

4 Publications Citing Use of MCE 3,4-Dicaffeoylquinic acid

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References
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Description

In Vitro

3,4-Dicaffeoylquinic acid acts as a greater primary antioxidant than its methanol extract, by having higher ferric reducing activity (EC₅₀ 2.18 μg/ml), β-carotene bleaching activity (EC₅₀ 23.85 μg/ml) and DPPH scavenging activity (EC₅₀ 68.91 μg/ml). 3,4-Dicaffeoylquinic acid exhibits a remarkable dose-dependent inhibitory effect on NCI-H23 (human lung adenocarcinoma) cell lines (EC₅₀ 3.26 μg/ml) and is found to be apoptotic in nature based on a clear indication of DNA fragmentation. 3,4-Dicaffeoylquinic acid also displays a concentration-dependent α-glucosidase inhibition with EC₅₀ 241.80 μg/ml^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

516.45

Formula

C₂₅H₂₄O₁₂

CAS No.

14534-61-3

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C([C@@]1(O)C[C@@H](OC(/C=C/C2=CC=C(O)C(O)=C2)=O)[C@H](OC(/C=C/C3=CC=C(O)C(O)=C3)=O)[C@H](O)C1)O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Methanol : 83.33 mg/mL (161.35 mM; Need ultrasonic)

DMSO : 50 mg/mL (96.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9363 mL	9.6815 mL	19.3630 mL
5 mM	0.3873 mL	1.9363 mL	3.8726 mL
10 mM	0.1936 mL	0.9681 mL	1.9363 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.64%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (254 KB) HNMR (295 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Takemura T, et al. 3,4-Dicaffeoylquinic Acid, a Major Constituent of Brazilian Propolis, Increases TRAIL Expression and Extends the Lifetimes of Mice Infected with the Influenza A Virus. Evid Based Complement Alternat Med. 2012;2012:946867. [Content Brief]

[2]. Liu X, et al. Protective effect of isochlorogenic acid B on liver fibrosis in non-alcoholic steatohepatitis of mice. Basic Clin Pharmacol Toxicol. 2019;124(2):144-153. [Content Brief]

[3]. Ooi KL, et al. Cytotoxic, apoptotic and anti-α-glucosidase activities of 3,4-di-O-caffeoyl quinic acid, an antioxidant isolated from the polyphenolic-rich extract of Elephantopus mollis Kunth. J Ethnopharmacol. 2011;135(3):685-695. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Methanol	1 mM	1.9363 mL	9.6815 mL	19.3630 mL	48.4074 mL
	5 mM	0.3873 mL	1.9363 mL	3.8726 mL	9.6815 mL
	10 mM	0.1936 mL	0.9681 mL	1.9363 mL	4.8407 mL
	15 mM	0.1291 mL	0.6454 mL	1.2909 mL	3.2272 mL
	20 mM	0.0968 mL	0.4841 mL	0.9681 mL	2.4204 mL
	25 mM	0.0775 mL	0.3873 mL	0.7745 mL	1.9363 mL
	30 mM	0.0645 mL	0.3227 mL	0.6454 mL	1.6136 mL
	40 mM	0.0484 mL	0.2420 mL	0.4841 mL	1.2102 mL
	50 mM	0.0387 mL	0.1936 mL	0.3873 mL	0.9681 mL
	60 mM	0.0323 mL	0.1614 mL	0.3227 mL	0.8068 mL
	80 mM	0.0242 mL	0.1210 mL	0.2420 mL	0.6051 mL
Methanol	100 mM	0.0194 mL	0.0968 mL	0.1936 mL	0.4841 mL

3,4-Dicaffeoylquinic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3,4-Dicaffeoylquinic acid14534-61-33,4-Di-O-caffeoylquinic acid Isochlorogenic acid BGlucosidaseInfluenza VirusApoptosisEndogenous MetabolitenaturallyantioxidativeDNAprotectiveneuroprotectivehepatoprotectiveInhibitorinhibitorinhibit

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3,4-Dicaffeoylquinic acid (Synonyms: 3,4-Di-O-caffeoylquinic acid; Isochlorogenic acid B)

Customer Review

Other Forms of 3,4-Dicaffeoylquinic acid:

3,4-Dicaffeoylquinic acid Related Antibodies

4 Publications Citing Use of MCE 3,4-Dicaffeoylquinic acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

3,4-Dicaffeoylquinic acid Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

3,4-Dicaffeoylquinic acid Related Classifications

Keywords:

Your Recently Viewed Products:

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