3-O-Methyldopa (Synonyms: 3-Methoxy-L-tyrosine; 3-O-Methyl-L-DOPA)

Name: 3-O-Methyldopa
Brand: MedChemExpress
SKU: HY-113468A
Rating: 4.5 (1 reviews)

Cat. No.: HY-113468A Purity: 99.16%: Data Sheet
SDS

COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

3-O-Methyldopa (3-Methoxy-L-tyrosine) is a metabolite of L-DOPA (HY-N0304) that can cross the blood-brain barrier (BBB). 3-O-Methyldopa inhibits the astrocyte-mediated protective effect of L-DOPA (HY-N0304) on dopaminergic neurons. In addition, 3-O-Methyldopa has certain antidepressant and neuroprotective activities. 3-O-Methyldopa can be used in the research of nervous system diseases such as depression and Parkinson's disease.

For research use only. We do not sell to patients.

3-O-Methyldopa Chemical Structure

CAS No. : 300-48-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 3-O-Methyldopa:

1 Publications Citing Use of MCE 3-O-Methyldopa

•Ann Med. 2024 Dec;56(1):2417179. [Abstract]

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View All Isoforms

IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[2]

IL-6

IL-1β

In Vitro

3-O-Methyldopa (10-100 μM; 24 h) significantly reduces the number of mesencephalic dopaminergic neurons in primary cultured mesencephalic neurons^[1].
3-O-Methyldopa (10-100 μM; 24 h) inhibits the protective effect of L-DOPA (HY-N0304) on dopaminergic neurons in the mixed culture of mesencephalic neurons and striatal astrocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

3-O-Methyldopa (3 mg/kg; intraperitoneal injection; 10 days) has an improving effect in a mouse model of depression^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mice with chronic social defeat stress (CSDS) depression^[2]
Dosage:	3 mg/kg
Administration:	Intraperitoneal injection; 10 days
Result:	Significantly improve depressive-like behaviors. Significantly increased the sucrose preference of mice, shortened the immobility time in the forced swim test and tail suspension test, and increased the center distance in the open field test. Reversed the increased levels of IL-1β and IL-6 in the hippocampus of CSDS mice.

Molecular Weight

211.22

Formula

C₁₀H₁₃NO₄

CAS No.

300-48-1

Appearance

Solid

Color

White to light yellow

SMILES

N[C@@H](CC1=CC=C(O)C(OC)=C1)C(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 10 mg/mL (47.34 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7345 mL	23.6723 mL	47.3447 mL
5 mM	0.9469 mL	4.7345 mL	9.4689 mL
10 mM	0.4734 mL	2.3672 mL	4.7345 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 10 mg/mL (47.34 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Purity & Documentation

Purity: 99.16%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (247 KB) HNMR (236 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Masato Asanuma, et al. 3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of L-DOPA. BMC Neurosci. 2016 Jul 25;17(1):52. [Content Brief]

[2]. Zhong Q, et al. Limosilactobacillus-related 3-OMDP as a potential therapeutic target for depression. Ann Med. 2024 Dec;56(1):2417179. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.7345 mL	23.6723 mL	47.3447 mL	118.3617 mL
	5 mM	0.9469 mL	4.7345 mL	9.4689 mL	23.6723 mL
	10 mM	0.4734 mL	2.3672 mL	4.7345 mL	11.8362 mL
	15 mM	0.3156 mL	1.5782 mL	3.1563 mL	7.8908 mL
	20 mM	0.2367 mL	1.1836 mL	2.3672 mL	5.9181 mL
	25 mM	0.1894 mL	0.9469 mL	1.8938 mL	4.7345 mL
	30 mM	0.1578 mL	0.7891 mL	1.5782 mL	3.9454 mL
	40 mM	0.1184 mL	0.5918 mL	1.1836 mL	2.9590 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

3-O-Methyldopa Related Classifications

Neurological Disease Inflammation/Immunology

Keywords:

3-O-Methyldopa300-48-13-Methoxy-L-tyrosine 3-O-Methyl-L-DOPADrug DerivativeInterleukin RelatedDrug derivativeILL-DOPACOMTdopaminedecarboxylaseneurotoxiccompetitivelydepression modelInhibitorinhibitorinhibit

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3-O-Methyldopa (Synonyms: 3-Methoxy-L-tyrosine; 3-O-Methyl-L-DOPA)

Customer Review

Other Forms of 3-O-Methyldopa:

1 Publications Citing Use of MCE 3-O-Methyldopa

Featured Recommendations

•Related Screening Libraries:

View All Interleukin Related Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

3-O-Methyldopa Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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