2. Metabolic Enzyme/Protease
  3. Liposome
  4. 306-N16B

306-N16B is a selective lung-targeted lipid nanoparticle that reversibly targets lung endothelial cells and specific immune cells through selective adsorption of a protein corona mediated by differences in tail structure (such as fibrinogen β/γ chain). 306-N16B binds to specific plasma proteins in the blood to form a protein corona, which guides the particles to be enriched in the lungs, releases mRNA and promotes target cell gene expression, exerts efficient lung cell transfection activity, and can precisely regulate gene delivery of different cell types in the lungs (such as endothelial cells and macrophages). 306-N16B can be used in gene therapy technologies for hereditary lung diseases including pulmonary lymphangioleiomyomatosis (LAM), restoring tumor suppressor function by delivering Tsc2 mRNA, and can also be used for lung-specific mRNA vaccines and gene editing therapies.

For research use only. We do not sell to patients.

306-N16B Chemical Structure

306-N16B Chemical Structure

CAS No. : 2803699-70-7

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Based on 1 publication(s) in Google Scholar

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Description

306-N16B is a selective lung-targeted lipid nanoparticle that reversibly targets lung endothelial cells and specific immune cells through selective adsorption of a protein corona mediated by differences in tail structure (such as fibrinogen β/γ chain). 306-N16B binds to specific plasma proteins in the blood to form a protein corona, which guides the particles to be enriched in the lungs, releases mRNA and promotes target cell gene expression, exerts efficient lung cell transfection activity, and can precisely regulate gene delivery of different cell types in the lungs (such as endothelial cells and macrophages). 306-N16B can be used in gene therapy technologies for hereditary lung diseases including pulmonary lymphangioleiomyomatosis (LAM), restoring tumor suppressor function by delivering Tsc2 mRNA, and can also be used for lung-specific mRNA vaccines and gene editing therapies[1][2].

In Vivo

In the in vivo fluorescence imaging experiment of mice, 306-N16B (0.5 mg/kg mRNA; 6 h) is significantly enriched in the lungs and efficiently expressed luciferase, while the signals in other organs such as the liver and spleen were weak, proving its lung-targeted delivery ability[1].
In the Cre/LoxP gene editing experiment, 306-N16B (0.75 mg mRNA equiv./kg; 7 d) induces Ai14 mouse lung endothelial cell-specific tdTomato expression, and flow cytometry shows that the endothelial cell transfection rate reached 33.6%, which is significantly higher than that of epithelial cells (1.5%) and macrophages (1.9%)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

1471.57

Formula

C75H151N7O4S8
CAS No.
Appearance

Oil

Color

Colorless to off-white

SMILES

O=C(CCN(CCCN(CCCN(CCC(NCCSSCCCCCCCCCCCC)=O)CCC(NCCSSCCCCCCCCCCCC)=O)C)CCC(NCCSSCCCCCCCCCCCC)=O)NCCSSCCCCCCCCCCCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (67.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.6795 mL 3.3977 mL 6.7955 mL
5 mM 0.1359 mL 0.6795 mL 1.3591 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.27%

SDS (252 KB)

COA (241 KB) HNMR (343 KB) RP-HPLC (248 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.6795 mL 3.3977 mL 6.7955 mL 16.9887 mL
5 mM 0.1359 mL 0.6795 mL 1.3591 mL 3.3977 mL
10 mM 0.0680 mL 0.3398 mL 0.6795 mL 1.6989 mL
15 mM 0.0453 mL 0.2265 mL 0.4530 mL 1.1326 mL
20 mM 0.0340 mL 0.1699 mL 0.3398 mL 0.8494 mL
25 mM 0.0272 mL 0.1359 mL 0.2718 mL 0.6795 mL
30 mM 0.0227 mL 0.1133 mL 0.2265 mL 0.5663 mL
40 mM 0.0170 mL 0.0849 mL 0.1699 mL 0.4247 mL
50 mM 0.0136 mL 0.0680 mL 0.1359 mL 0.3398 mL
60 mM 0.0113 mL 0.0566 mL 0.1133 mL 0.2831 mL
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306-N16B Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

306-N16B2803699-70-7Liposome306-N16B is a lipidnanoparticleand allows systemic codelivery of Cas9 mRNA and sgRNA. 306-N16B can transport mRNA to the pulmonaryendothelial cell. 306-N16B can be used for research of genome editing-based therapies[1].Inhibitorinhibitorinhibit

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