4-Hydroxybenzoic acid

Name: 4-Hydroxybenzoic acid
Brand: MedChemExpress
SKU: HY-Y0264
Rating: 4.5 (1 reviews)

Cat. No.: HY-Y0264 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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4-Hydroxybenzoic acid, a phenolic derivative of benzoic acid, could inhibit most gram-positive and some gram-negative bacteria, with an IC₅₀ of 160 μg/mL.

For research use only. We do not sell to patients.

4-Hydroxybenzoic acid Chemical Structure

CAS No. : 99-96-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
1 g	USD 48	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of 4-Hydroxybenzoic acid:

4-Hydroxybenzoic acid-¹³C₆ Get quote
4-Hydroxybenzoic acid-d₄ Get quote
4-Hydroxybenzoic acid-¹³C Get quote
4-Hydroxybenzoic acid (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

4-Hydroxybenzoic acid, a phenolic derivative of benzoic acid, could inhibit most gram-positive and some gram-negative bacteria, with an IC₅₀ of 160 μg/mL.

IC₅₀ & Target^[1]

Microbial Metabolite

Bacteria

160 μg/mL (IC₅₀)

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: 15	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 21696954]
A549	IC₅₀	> 100 μM Compound: 15	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 19689125]
B16-BL6	IC₅₀	63.3 μM Compound: 15	Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 19689125]
BV-2	IC₅₀	> 100 μM Compound: 25	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 28911817]
HEK293	EC₅₀	1.11 nM Compound: PHBA	Positive allosteric modulation of human D1R expressed in HEK293 cells assessed as increase in dopamine-induced cAMP accumulation after 60 mins by HTRF assay Positive allosteric modulation of human D1R expressed in HEK293 cells assessed as increase in dopamine-induced cAMP accumulation after 60 mins by HTRF assay	[PMID: 30525590]
HeLa	IC₅₀	36.2 μM Compound: 15	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 19689125]
HT-1080	IC₅₀	> 100 μM Compound: 15	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 19689125]
HT-22	EC₅₀	> 100 μM Compound: 34	Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay	[PMID: 32991171]
KB	IC₅₀	> 200 μM Compound: 1e	Antitumor activity against KB cells by MTT assay Antitumor activity against KB cells by MTT assay	[PMID: 17387015]
LoVo	IC₅₀	94 μM Compound: 15	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 21696954]
N9	IC₅₀	> 100 μM Compound: 32	Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 28073678]
PC-3	IC₅₀	> 100 μM Compound: 15	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 21696954]
RAW264.7	IC₅₀	118.11 μM Compound: 3c	Antagonist activity at TLR2 in mouse RAW264.7 cells assessed as PAM3CSK4-induced NO production measured after 24 hrs by Griess assay Antagonist activity at TLR2 in mouse RAW264.7 cells assessed as PAM3CSK4-induced NO production measured after 24 hrs by Griess assay	[PMID: 31255924]
RAW264.7	IC₅₀	72.8 μM Compound: 8	Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay	[PMID: 25666824]
SK-MEL-28	IC₅₀	> 100 μM Compound: 15	Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 21696954]
U-373MG ATCC	IC₅₀	> 100 μM Compound: 15	Cytotoxicity against human U373 cells after 72 hrs by MTT assay Cytotoxicity against human U373 cells after 72 hrs by MTT assay	[PMID: 21696954]

In Vitro

Most of the gram-positive and some gram-negative bacteria are sensitive to trans 4-Hydroxycinnamic acid (4-HBA) and 4-Hydroxybenzoic acid at IC₅₀ concentrations of 100-170 and 160 μg/mL, respectively. The antimicrobial activities of 4-Hydroxycinnamic acid and t4-HCA against 11 food pathogenic bacteria, 6 plant pathogenic bacteria, 2 yeasts and 15 plant pathogenic fungi are tested by the paper disc method. These compounds inhibit the growth of most of the bacteria and yeasts at concentrations of 200-400 μg. However, the inhibition is more effective against most of the gram-positive bacteria. When tested by the paper disc method, 4-Hydroxycinnamic acid has stronger antimicrobial activity than t4-HCA against S. aureus, L. mesenteroides, S. cerevisiae and C. albicans at a concentration of 50 μg. However, no inhibitory effect against fungi was observed at concentrations even up to 1000 μg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

138.12

Formula

C₇H₆O₃

CAS No.

99-96-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C1=CC=C(O)C=C1

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 120 mg/mL (868.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.2400 mL	36.1999 mL	72.3998 mL
5 mM	1.4480 mL	7.2400 mL	14.4800 mL
10 mM	0.7240 mL	3.6200 mL	7.2400 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3 mg/mL (21.72 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3 mg/mL (21.72 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.86 mg/mL (20.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.86 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (28.6 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (271 KB) SDS (614 KB)

COA (246 KB)

Handling Instructions (2659 KB)

References

[1]. Cho JY, Antimicrobial activity of 4-hydroxybenzoic acid and trans 4-hydroxycinnamic acid isolated and identified from rice hull. Biosci Biotechnol Biochem. 1998 Nov;62(11):2273-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	7.2400 mL	36.1999 mL	72.3998 mL	180.9994 mL
	5 mM	1.4480 mL	7.2400 mL	14.4800 mL	36.1999 mL
	10 mM	0.7240 mL	3.6200 mL	7.2400 mL	18.0999 mL
	15 mM	0.4827 mL	2.4133 mL	4.8267 mL	12.0666 mL
	20 mM	0.3620 mL	1.8100 mL	3.6200 mL	9.0500 mL
	25 mM	0.2896 mL	1.4480 mL	2.8960 mL	7.2400 mL
	30 mM	0.2413 mL	1.2067 mL	2.4133 mL	6.0333 mL
	40 mM	0.1810 mL	0.9050 mL	1.8100 mL	4.5250 mL
	50 mM	0.1448 mL	0.7240 mL	1.4480 mL	3.6200 mL
	60 mM	0.1207 mL	0.6033 mL	1.2067 mL	3.0167 mL
	80 mM	0.0905 mL	0.4525 mL	0.9050 mL	2.2625 mL
	100 mM	0.0724 mL	0.3620 mL	0.7240 mL	1.8100 mL