4-Hydroxymethylpyrazole

Name: 4-Hydroxymethylpyrazole
Brand: MedChemExpress
SKU: HY-33914
Rating: 4.5 (1 reviews)

Cat. No.: HY-33914 Purity: 99.74%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-Hydroxymethylpyrazole is the primary metabolite of Fomepizole (HY-B0876). Fomepizole is a competitive inhibitor of the enzyme alcohol dehydrogenase.

For research use only. We do not sell to patients.

4-Hydroxymethylpyrazole Chemical Structure

CAS No. : 25222-43-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 125	In-stock	Estimated Time of Arrival: December 31
1 g	USD 190	In-stock	Estimated Time of Arrival: December 31
5 g	USD 400	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

4-Hydroxymethylpyrazole is the primary metabolite of Fomepizole (HY-B0876). Fomepizole is a competitive inhibitor of the enzyme alcohol dehydrogenase^[1].

Molecular Weight

98.10

Formula

C₄H₆N₂O

CAS No.

25222-43-9

Appearance

Solid

Color

White to off-white

SMILES

OCC1=CNN=C1

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (1019.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (1019.37 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	10.1937 mL	50.9684 mL	101.9368 mL
5 mM	2.0387 mL	10.1937 mL	20.3874 mL
10 mM	1.0194 mL	5.0968 mL	10.1937 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (25.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (25.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (25.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (245 KB) HNMR (129 KB) RP-HPLC (227 KB)

Handling Instructions (2659 KB)

References

[1]. R Blomstrand, et al. Pyrazoles as Inhibitors of Alcohol Oxidation and as Important Tools in Alcohol Research: An Approach to Therapy Against Methanol Poisoning. Proc Natl Acad Sci U S A . 1979 Jul;76(7):3499-503. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	10.1937 mL	50.9684 mL	101.9368 mL	254.8420 mL
	5 mM	2.0387 mL	10.1937 mL	20.3874 mL	50.9684 mL
	10 mM	1.0194 mL	5.0968 mL	10.1937 mL	25.4842 mL
	15 mM	0.6796 mL	3.3979 mL	6.7958 mL	16.9895 mL
	20 mM	0.5097 mL	2.5484 mL	5.0968 mL	12.7421 mL
	25 mM	0.4077 mL	2.0387 mL	4.0775 mL	10.1937 mL
	30 mM	0.3398 mL	1.6989 mL	3.3979 mL	8.4947 mL
	40 mM	0.2548 mL	1.2742 mL	2.5484 mL	6.3711 mL
	50 mM	0.2039 mL	1.0194 mL	2.0387 mL	5.0968 mL
	60 mM	0.1699 mL	0.8495 mL	1.6989 mL	4.2474 mL
	80 mM	0.1274 mL	0.6371 mL	1.2742 mL	3.1855 mL
	100 mM	0.1019 mL	0.5097 mL	1.0194 mL	2.5484 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.