4-tert-Octylphenol

Name: 4-tert-Octylphenol
Brand: MedChemExpress
SKU: HY-B1941
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1941 Purity: 99.48%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-tert-Octylphenol, a endocrine-disrupting chemical, is an estrogenic agent. 4-tert-Octylphenol is also a biodegradation product of non-ionic surfactants alkylphenol polyethoxylates. 4-tert-Octylphenol induces apoptosis in neuronal progenitor cells in offspring mouse brain. 4-tert-Octylphenol reduces bromodeoxyuridine (BrdU), mitotic marker Ki67, and phospho-histone H3 (p-Histone-H3), resulting in a reduction of neuronal progenitor proliferation. 4-tert-Octylphenol disrupts brain development and behavior in mice, which is promising for reserch of immune response, neuro-related diseases and ethology.

For research use only. We do not sell to patients.

4-tert-Octylphenol Chemical Structure

CAS No. : 140-66-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
10 g	USD 30	Get quote	Estimated Time of Arrival: December 31
25 g	USD 50	Get quote	Estimated Time of Arrival: December 31
50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 4-tert-Octylphenol:

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

4-tert-Octylphenol (0.01 and 1 μM, 24 h) may inhibit proliferation and promote apoptosis of neuronal progenitor cells during the early stage of brain development^[1].
4-tert-Octylphenol (10 μM, 6 h) down-regulates the expression of IL-12p35, IFN-γ2 and CXCb2 in LPS-stimulated monocytes/macrophages and decreases the levels of nitric oxide (NO) released from LPS-stimulated cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:	Primary cortical neurons
Concentration:	0.01 and 1 μM
Incubation Time:	24 h
Result:	The numbers of primary and secondary dendrites in the 4-tert-Octylphenol-treated groups were markedly higher than those in the vehicle group in neuron cells.

Immunofluorescence^[3]

Cell Line:	LPS-stimulated monocytes/macrophages
Concentration:	0.1, 1, 10 μM
Incubation Time:	6 h
Result:	Down-regulated the expression of IL-12p35, IFN-γ2 and CXCb2 in LPS-stimulated monocytes/macrophages and decreased the levels of NO released from LPS-stimulated cells.

Cell Proliferation Assay^[1]

Cell Line:	Primary cortical neuronal cells
Concentration:	0.01 and 1 μM
Incubation Time:	12 h
Result:	The percentage of BrdU⁺ cells in the OP 1 μM group was significantly lower than that in the OP 0.01 μM group in primary cortical neuronal cells.

In Vivo

4-tert-Octylphenol (10, 50 mg/kg, s.c., a single dose) promotes cell death in offspring mouse brain, induces cognitive dysfunction and impairs sociability and decreases social novelty preference in offspring mice^[1].
4-tert-Octylphenol (10, 50 mg/kg, s.c., a single dose for 24 h) promotes cell cycle exit and inhibits cell cycle reentry of dentate gyrus (DG) neural progenitors in embryonic and adult neurogenesis^[1].
4-tert-Octylphenol (2.5 μg/kg, feed, daily for 14 days) lowers numbers of peritoneal leukocytes/phagocytes compared to those in control infected animals in peritoneal and head kidney leukocytes of fish^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Pathogen-free C57BL/6J male and female mice (8 weeks old, 25-30 g)^[1]
Dosage:	10, 50 mg/kg
Administration:	s.c., a single dose
Result:	Elevated apoptosis in offspring mouse brain and impaired cognitive functioning in areas such as spatial and nonspatial learning and memory.

Animal Model:	Sexually immature, young (9–12 months) common carp^[3]
Dosage:	2.5 μg/kg food
Administration:	feed, daily for 14 days
Result:	Lowed numbers of peritoneal leukocytes/phagocytes in peritoneal and head kidney leukocytes of fish.

Molecular Weight

206.32

Formula

C₁₄H₂₂O

CAS No.

140-66-9

Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=C(C(C)(C)CC(C)(C)C)C=C1

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (484.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8468 mL	24.2342 mL	48.4684 mL
5 mM	0.9694 mL	4.8468 mL	9.6937 mL
10 mM	0.4847 mL	2.4234 mL	4.8468 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.48%

Select Batch:

Data Sheet (278 KB) SDS (560 KB)

COA (246 KB) HNMR (111 KB) RP-HPLC (199 KB)

Handling Instructions (2659 KB)

References

[1]. Dinh Nam Tran, et al. 4-tert-Octylphenol Exposure Disrupts Brain Development and Subsequent Motor, Cognition, Social, and Behavioral Functions. Oxidative Medicine and Cellular Longevity, 2020.

[2]. Olaniyan LWB, et al. Environmental Water Pollution, Endocrine Interference and Ecotoxicity of 4-tert-Octylphenol: A Review[J]. Rev Environ Contam Toxicol. 2020;248:81-109. [Content Brief]

[3]. Maciuszek M, et al. 17α-ethinylestradiol and 4-tert-octylphenol concurrently disrupt the immune response of common carp[J]. Fish Shellfish Immunol. 2020 Dec;107(Pt A):238-250. [Content Brief]

[4]. Lee J, Zee S, et al. Effects of crosstalk between steroid hormones mediated thyroid hormone in zebrafish exposed to 4-tert-octylphenol: Estrogenic and anti-androgenic effects[J]. Ecotoxicol Environ Saf. 2024 Jun 1;277:116348. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.8468 mL	24.2342 mL	48.4684 mL	121.1710 mL
	5 mM	0.9694 mL	4.8468 mL	9.6937 mL	24.2342 mL
	10 mM	0.4847 mL	2.4234 mL	4.8468 mL	12.1171 mL
	15 mM	0.3231 mL	1.6156 mL	3.2312 mL	8.0781 mL
	20 mM	0.2423 mL	1.2117 mL	2.4234 mL	6.0585 mL
	25 mM	0.1939 mL	0.9694 mL	1.9387 mL	4.8468 mL
	30 mM	0.1616 mL	0.8078 mL	1.6156 mL	4.0390 mL
	40 mM	0.1212 mL	0.6059 mL	1.2117 mL	3.0293 mL
	50 mM	0.0969 mL	0.4847 mL	0.9694 mL	2.4234 mL
	60 mM	0.0808 mL	0.4039 mL	0.8078 mL	2.0195 mL
	80 mM	0.0606 mL	0.3029 mL	0.6059 mL	1.5146 mL
	100 mM	0.0485 mL	0.2423 mL	0.4847 mL	1.2117 mL