5-Methyl-2'-deoxycytidine (Synonyms: 5-Methyldeoxycytidine)

Name: 5-Methyl-2'-deoxycytidine
Brand: MedChemExpress
SKU: HY-W012078
Rating: 4.5 (1 reviews)

Cat. No.: HY-W012078 Purity: 99.87%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5-Methyl-2'-deoxycytidine in single-stranded DNA can act in cis to signal de novo DNA methylation.

For research use only. We do not sell to patients.

5-Methyl-2'-deoxycytidine Chemical Structure

CAS No. : 838-07-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 45	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 5-Methyl-2'-deoxycytidine:

5-Methyl-2'-deoxycytidine-d₃ Get quote

1 Publications Citing Use of MCE 5-Methyl-2'-deoxycytidine

•Nucleic Acids Res. 2021 Oct 29;gkab989. [Abstract]

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All DNA Methyltransferase Isoform Specific Products:

View All Isoforms

DNA Methyltransferase DNMT1 DNMT3 MGMT

Biological Activity
Purity & Documentation
References
Customer Review

Description

5-Methyl-2'-deoxycytidine in single-stranded DNA can act in cis to signal de novo DNA methylation^[1]^[2].

IC₅₀ & Target

Human Endogenous Metabolite

Molecular Weight

241.24

Formula

C₁₀H₁₅N₃O₄

CAS No.

838-07-3

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](O)C[C@H](N2C(N=C(N)C(C)=C2)=O)O1

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (414.52 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (414.52 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1452 mL	20.7262 mL	41.4525 mL
5 mM	0.8290 mL	4.1452 mL	8.2905 mL
10 mM	0.4145 mL	2.0726 mL	4.1452 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (414.52 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (252 KB) HNMR (124 KB) RP-HPLC (318 KB)

Handling Instructions (2659 KB)

References

[1]. Christman JK, et al. 5-Methyl-2'-deoxycytidine in single-stranded DNA can act in cis to signal de novo DNA methylation. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7347-51. [Content Brief]

[2]. Testillano PS, et al. The 5-methyl-deoxy-cytidine (5mdC) localization to reveal in situ the dynamics of DNA methylation chromatin pattern in a variety of plant organ and tissue cells during development. Physiol Plant. 2013 Sep;149(1):104-13. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.1452 mL	20.7262 mL	41.4525 mL	103.6312 mL
	5 mM	0.8290 mL	4.1452 mL	8.2905 mL	20.7262 mL
	10 mM	0.4145 mL	2.0726 mL	4.1452 mL	10.3631 mL
	15 mM	0.2763 mL	1.3817 mL	2.7635 mL	6.9087 mL
	20 mM	0.2073 mL	1.0363 mL	2.0726 mL	5.1816 mL
	25 mM	0.1658 mL	0.8290 mL	1.6581 mL	4.1452 mL
	30 mM	0.1382 mL	0.6909 mL	1.3817 mL	3.4544 mL
	40 mM	0.1036 mL	0.5182 mL	1.0363 mL	2.5908 mL
	50 mM	0.0829 mL	0.4145 mL	0.8290 mL	2.0726 mL
	60 mM	0.0691 mL	0.3454 mL	0.6909 mL	1.7272 mL
	80 mM	0.0518 mL	0.2591 mL	0.5182 mL	1.2954 mL
	100 mM	0.0415 mL	0.2073 mL	0.4145 mL	1.0363 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

5-Methyl-2'-deoxycytidine Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Methyl-2'-deoxycytidine838-07-35-MethyldeoxycytidineDNA MethyltransferaseEndogenous MetaboliteDNMTsDNA MTasesInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

5-Methyl-2'-deoxycytidine (Synonyms: 5-Methyldeoxycytidine)

Customer Review

Other Forms of 5-Methyl-2'-deoxycytidine:

5-Methyl-2'-deoxycytidine Related Antibodies

1 Publications Citing Use of MCE 5-Methyl-2'-deoxycytidine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All DNA Methyltransferase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

5-Methyl-2'-deoxycytidine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report