6-(Methylthio)purine (Synonyms: 6-Methylmercaptopurine)

Cat. No.: HY-W097453 Purity: 97.04%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

6-(Methylthio)purine (6-Methylmercaptopurine) is a small molecule that can be used for compound synthesis.

For research use only. We do not sell to patients.

6-(Methylthio)purine Chemical Structure

CAS No. : 50-66-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	Get quote	Estimated Time of Arrival: December 31
500 mg	Get quote
1 g	Get quote

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Other Forms of 6-(Methylthio)purine:

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Biological Activity
Purity & Documentation
References
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Description

6-(Methylthio)purine (6-Methylmercaptopurine) is a small molecule that can be used for compound synthesis^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	23.6 μM Compound: 10	Antiproliferative activity against human MCF7 cells after 3 days by SRB assay Antiproliferative activity against human MCF7 cells after 3 days by SRB assay	[PMID: 18069093]

Molecular Weight

166.21

Formula

C₆H₆N₄S

CAS No.

50-66-8

Appearance

Solid

Color

White to off-white

SMILES

CSC1=NC=NC2=C1N=CN2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (601.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.0165 mL	30.0826 mL	60.1652 mL
5 mM	1.2033 mL	6.0165 mL	12.0330 mL
10 mM	0.6017 mL	3.0083 mL	6.0165 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 97.04%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (253 KB) HNMR (117 KB) RP-HPLC (94 KB)

Handling Instructions (2659 KB)

References

[1]. Sugimura H, Takei H. Synthesis of 6-alkylpurine derivatives by nickel-complex-catalyzed coupling reaction of 6-(methylthio) purine derivatives with Grignard reagents. Bulletin of the Chemical Society of Japan, 1985, 58(2): 664-666.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.0165 mL	30.0826 mL	60.1652 mL	150.4130 mL
	5 mM	1.2033 mL	6.0165 mL	12.0330 mL	30.0826 mL
	10 mM	0.6017 mL	3.0083 mL	6.0165 mL	15.0413 mL
	15 mM	0.4011 mL	2.0055 mL	4.0110 mL	10.0275 mL
	20 mM	0.3008 mL	1.5041 mL	3.0083 mL	7.5207 mL
	25 mM	0.2407 mL	1.2033 mL	2.4066 mL	6.0165 mL
	30 mM	0.2006 mL	1.0028 mL	2.0055 mL	5.0138 mL
	40 mM	0.1504 mL	0.7521 mL	1.5041 mL	3.7603 mL
	50 mM	0.1203 mL	0.6017 mL	1.2033 mL	3.0083 mL
	60 mM	0.1003 mL	0.5014 mL	1.0028 mL	2.5069 mL
	80 mM	0.0752 mL	0.3760 mL	0.7521 mL	1.8802 mL
	100 mM	0.0602 mL	0.3008 mL	0.6017 mL	1.5041 mL

6-(Methylthio)purine Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-(Methylthio)purine50-66-86-MethylmercaptopurineEndogenous MetaboliteInhibitorinhibitorinhibit

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